LNCaP nuclear extract lysate (testosterone treated) (ab14858)

Overview

  • Product name

    LNCaP nuclear extract lysate (testosterone treated)
    See all LNCaP lysates
  • General notes

    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Cells were cultured in medium supplemented with 10 nM Testosterone for 6 hours at 37°C immediately prior to harvesting.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas

  • Background

    The LNCaP-FGC (fast growing colony) cell line is a subline derived from the LNCaP cell line, sharing all the main characteristics, including its androgen sensitivity, described for the parental line. These fibroblast-like cells express high levels of human prostatic acid phosphatase, androgen receptors and prostate antigen. They are used as a model of prostate cancer especially in studies of the development of androgen resistance in human prostate cancer.

References

ab14858 has not yet been referenced specifically in any publications.

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