Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab21203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).|
FunctionLinks T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase.
Tissue specificityPreferentially expressed by lymphoid cell lines.
Involvement in diseaseGenetic variations in SH2B3 are associated with susceptibility to celiac disease type 13 (CELIAC13)[MIM:612011]; also known as susceptibility to gluten-sensitive enteropathy type 13. Celiac disease is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive.
Genetic variations in SH2B3 are associated with susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100]; also known as diabetes mellitus type 1. IDDM normally starts in childhood or adolescence and is caused by the body's own immune system which destroys the insulin-producing beta cells in the pancreas. Classical features are polydipsia, polyphagia and polyuria, due to hyperglycemia-induced osmotic diuresis.
Sequence similaritiesBelongs to the SH2B adapter family.
Contains 1 PH domain.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated by LCK.
- Information by UniProt
- IDDM20 antibody
- Lymphocyte adapter protein antibody
- Lymphocyte specific adapter protein antibody
Anti-LNK antibody (ab21203) at 0.3 µg/ml + MOLT4 lysate (35µg protein in RIPA buffer).
Developed using the ECL technique.
Predicted band size: 63 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
g/ml) staining of MOLT4 lysate (35 µ g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. µ