Key features and details
- Rabbit polyclonal to LNK
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LNK antibody
See all LNK primary antibodies
DescriptionRabbit polyclonal to LNK
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- WB: HEL cell lysate. IHC-P: Human lymph node tissue. ICC/IF U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 63 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionLinks T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase.
Tissue specificityPreferentially expressed by lymphoid cell lines.
Involvement in diseaseGenetic variations in SH2B3 are associated with susceptibility to celiac disease type 13 (CELIAC13)[MIM:612011]; also known as susceptibility to gluten-sensitive enteropathy type 13. Celiac disease is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive.
Genetic variations in SH2B3 are associated with susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100]; also known as diabetes mellitus type 1. IDDM normally starts in childhood or adolescence and is caused by the body's own immune system which destroys the insulin-producing beta cells in the pancreas. Classical features are polydipsia, polyphagia and polyuria, due to hyperglycemia-induced osmotic diuresis.
Sequence similaritiesBelongs to the SH2B adapter family.
Contains 1 PH domain.
Contains 1 SH2 domain.
modificationsTyrosine phosphorylated by LCK.
- Information by UniProt
- IDDM20 antibody
- Lymphocyte adapter protein antibody
- Lymphocyte specific adapter protein antibody
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for LNK (green) using ab244278 at 4 µg/ml in ICC/IF.
Formalin-fixed, paraffin-embedded human lymph node tissue stained for LNK using ab244278 at 1/20 dilution in immunohistochemical analysis.
Anti-LNK antibody (ab244278) at 0.4 µg/ml + HEL (human erythroleukemia cell line) cell lysate
Predicted band size: 63 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244278 has not yet been referenced specifically in any publications.