Key features and details
- Rabbit polyclonal to LNPEP - N-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LNPEP antibody - N-terminal
See all LNPEP primary antibodies
DescriptionRabbit polyclonal to LNPEP - N-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Pig, Chimpanzee, Rhesus monkey, Common marmoset
- HeLa, 293T and Jurkat whole cell lysates.
Previously labelled as Leucyl-cystinyl aminopeptidase.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab190255 was affinity purified using an epitope specific to LNPEP immobilized on solid support.
Our Abpromise guarantee covers the use of ab190255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 117 kDa.|
FunctionRelease of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain.
Tissue specificityHighly expressed in placenta, heart, kidney and small intestine. Detected at lower levels in neuronal cells in the brain, in skeletal muscle, spleen, liver, testes and colon.
Sequence similaritiesBelongs to the peptidase M1 family.
modificationsThe pregnancy serum form is derived from the membrane-bound form by proteolytic processing.
Cellular localizationCell membrane. In brain only the membrane-bound form is found. The protein resides in intracellular vesicles together with GLUT4 and can then translocate to the cell surface in response to insulin and/or oxytocin. Localization may be determined by dileucine internalization motifs, and/or by interaction with tankyrases and Secreted. During pregnancy serum levels are low in the first trimester, rise progressively during the second and third trimester and decrease rapidly after parturition.
- Information by UniProt
- angiotensin IV receptor antibody
- AT (4) receptor antibody
- CAP antibody
Detection of LNPEP by Western Blot of Immunoprecipitate.
ab190255 at 1 µg/ml labeling LNPEP in Jurkat whole cell lysate immunoprecipitated using ab190255 at 6 µg/reaction (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-LNPEP antibody - N-terminal (ab190255) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 117 kDa
Exposure time: 3 minutes
ab190255 has not yet been referenced specifically in any publications.