Key features and details
- Mouse monoclonal [1H9] to Lnx1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Lnx1 antibody [1H9]
See all Lnx1 primary antibodies
DescriptionMouse monoclonal [1H9] to Lnx1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Lnx1 aa 260-528. (NP_116011) Produced in E. coli.
Database link: Q8TBB1
- IHC-P: Human brain cortex tissue. WB: pCMV6-ENTRY cDNA Lnx1 transfected cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab230704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml.|
|WB||1/500. Predicted molecular weight: 81 kDa.|
RelevanceLnx1 is a membrane-bound protein that is involved in signal transduction and protein interactions. Lnx1 is an E3 Ubiquitin ligase protein that mediates ubiquitination and subsequent proteasomal degradation of proteins containing phosphotyrosine binding (PTB) domains. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Lnx1 mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65.
- E3 ubiquitin protein ligase LNX antibody
- Ligand of Numb protein X 1 antibody
- Ligand of numb protein X 1 E3 ubiquitin protein ligase antibody
All lanes : Anti-Lnx1 antibody [1H9] (ab230704) at 1/500 dilution
Lane 1 : pCMV6-ENTRY cDNA control transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY cDNA Lnx1 transfected HEK-293T whole cell lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 81 kDa
Formalin-fixed, paraffin-embedded human brain cortex tissue stained for Lnx1 with ab230704 at 10 µg/ml in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230704 has not yet been referenced specifically in any publications.