Overview

  • Product name

    Anti-Lnx1 antibody [1H9]
    See all Lnx1 primary antibodies
  • Description

    Mouse monoclonal [1H9] to Lnx1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Lnx1 aa 260-528. (NP_116011) Produced in E. coli.
    Database link: Q8TBB1

  • Positive control

    • IHC-P: Human brain cortex tissue. WB: pCMV6-ENTRY cDNA Lnx1 transfected cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    1H9
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB 1/500. Predicted molecular weight: 81 kDa.

Target

  • Relevance

    Lnx1 is a membrane-bound protein that is involved in signal transduction and protein interactions. Lnx1 is an E3 Ubiquitin ligase protein that mediates ubiquitination and subsequent proteasomal degradation of proteins containing phosphotyrosine binding (PTB) domains. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Lnx1 mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • E3 ubiquitin protein ligase LNX antibody
    • Ligand of Numb protein X 1 antibody
    • Ligand of numb protein X 1 E3 ubiquitin protein ligase antibody
    • LNX antibody
    • LNX1 antibody
    • MPDZ antibody
    • Multi PDZ domain containing protein E3 ubiquitin protein ligase LNX antibody
    • Numb binding protein 1 antibody
    • PDZ domain-containing ring finger protein 2 antibody
    • PDZRN2 antibody
    see all

Images

  • All lanes : Anti-Lnx1 antibody [1H9] (ab230704) at 1/500 dilution

    Lane 1 : pCMV6-ENTRY cDNA control transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : pCMV6-ENTRY cDNA Lnx1 transfected HEK-293T whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 81 kDa

  • Formalin-fixed, paraffin-embedded human brain cortex tissue stained for Lnx1 with ab230704 at 10 µg/ml in immunohistochemical analysis.

References

ab230704 has not yet been referenced specifically in any publications.

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