Overview

  • Product name
    Anti-LNX2 antibody - C-terminal
    See all LNX2 primary antibodies
  • Description
    Rabbit polyclonal to LNX2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LNX2 aa 550-579 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8N448

  • Positive control
    • HepG2, 293 and NCI-H460 cell line lysates, Human rectum tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171454 is purified through a Protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 76 kDa.

Target

  • Sequence similarities
    Contains 4 PDZ (DHR) domains.
    Contains 1 RING-type zinc finger.
  • Domain
    The NPXY motif is required for the interaction with the PID domain of NUMB. It is however not sufficient.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ12933 antibody
    • FLJ23932 antibody
    • FLJ38000 antibody
    • Ligand of Numb protein X 2 antibody
    • LNX 2 antibody
    • LNX2 antibody
    • LNX2_HUMAN antibody
    • MGC46315 antibody
    • Numb binding protein 2 antibody
    • Numb-binding protein 2 antibody
    • PDZ domain containing ring finger 1 antibody
    • PDZ domain containing RING finger protein 1 antibody
    • PDZ domain-containing RING finger protein 1 antibody
    • PDZRN 1 antibody
    • PDZRN1 antibody
    see all

Images

  • All lanes : Anti-LNX2 antibody - C-terminal (ab171454) at 1/100 dilution

    Lane 1 : HepG2 cell line lysate
    Lane 2 : 293 cell line lysate
    Lane 3 : NCI-H460 cell line lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 76 kDa

  • Immunohistochemical analysis of paraffin embedded Human rectum tissue labeling LNX2 with ab171454 at 1/10 followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab171454 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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