Key features and details
- Rabbit polyclonal to LOC134147/CMBL
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-LOC134147/CMBL antibody
See all LOC134147/CMBL primary antibodies
DescriptionRabbit polyclonal to LOC134147/CMBL
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Recombinant full length protein corresponding to Human LOC134147/CMBL aa 2-245.
ANEAYPCPCDIGHRLEYGGLGREVQVEHIKAYVTKSPVDAGKAVIVIQDI FGWQLPNTRYIADMISGNGYTTIVPDFFVGQEPWDPSGDWSIFPEWLKTR NAQKIDREISAILKYLKQQCHAQKIGIVGFCWGGTAVHHLMMKYSEFRAG VSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVE YQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLNKYM
Database link: Q96DG6
- WB: Mouse kidney and liver tissue lysate. IHC-P: Human kidney and small intestine tissue. ICC/IF: HeLa cells.
This product was previously labelled as LOC134147
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 28 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionCysteine hydrolase. Can convert the prodrug olmesartan medoxomil into its pharmacologically active metabolite olmerstatan, an angiotensin receptor blocker, in liver and intestine. May also activate beta-lactam antibiotics faropenem medoxomil and lenampicillin.
Tissue specificityWidely expressed, with highest levels in liver, followed by kidney, small intestine and colon. Present in liver and intestine (at protein level).
Sequence similaritiesBelongs to the dienelactone hydrolase family.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- Carboxymethylenebutenolidase homolog (Pseudomonas) antibody
- Carboxymethylenebutenolidase homolog antibody
- cmbl antibody
All lanes : Anti-LOC134147/CMBL antibody (ab237694) at 1/500 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse liver tissue lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 28 kDa
Paraffin-embedded human kidney tissue stained for LOC134147/CMBL with ab237694 at a 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for LOC134147/CMBL with ab237694 at a 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for LOC134147/CMBL (Green) using ab237694 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237694 has not yet been referenced specifically in any publications.