Anti-Lok antibody (ab136852)

This fast track antibody is not yet fully characterized. It is subject to these terms and conditions.

Overview

  • Product name

    Anti-Lok antibody
  • Description

    Goat polyclonal to Lok

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species

    Goat
  • Species reactivity


    Predicted to work with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide:

    C-QDLSRNGTFVNNEK

    , corresponding to internal sequence amino acids 115-128 of Fruit fly (Drosophila melanogaster) Lok (NP_477219.1; NP_477218.1; NP_724241.1).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7,30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab136852 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB Use at an assay dependent concentration. ab136852 did not give bands in initial tests on lysate/cell line. We would welcome your feedback if you have tested this antibody in more suitable positive controls.

Target

  • Function

    May have a role in germline establishment.
  • Tissue specificity

    In stage 3 embryos, both isoforms are expressed in both somatic and pole cell nuclei. Expression in pole cell nuclei is sustained until stage 9 and weakly expressed after pole cell invagination into the abdominal cavity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CDS1 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.
  • Developmental stage

    Expressed both maternally and zygotically in adult females. Levels of the long isoform remain fairly constant from ovaries to embryos, the levels of short isoform decrease dramatically.
  • Cellular localization

    Nucleus speckle. Speckled subnuclear compartment.
  • Information by UniProt
  • Database links

    • Alternative names

      • dMNK antibody
      • lok antibody
      • LOK_DROME antibody
      • Ovarian-specific serine/threonine-protein kinase Lok antibody
      • Protein loki antibody
      see all

    References

    ab136852 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (4-20% gel)
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryo)
    Specification
    0-4hr embryo
    Treatment
    control shRNA and lok shRNA embryos
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

    Dr. Richelle Sopko

    Verified customer

    Submitted Aug 02 2013

    Answer

    Thank you for contacting us.

    AB136852 Anti-Lok antibody is a Fast-Track antibody. These products are not eligible for the AbTrial program. We offer an enhanced Feedback Reward for these products in lieu of the AbTrial program. I have included more details about our Fast Track products below. Please do not hesitate to contact us should you have any questions.

    Fast-Track antibodies constitute a diverse group of products that have undergone variable degrees of characterization. They have all been affinity purified and show high titer values against the immunizing peptide (by ELISA).

    As a general rule, Fast-Track antibodies fall into two main categories:

    · Cutting-edge products: these are released quickly to meet customer needs and requirements; the products are still undergoing further tests and characterization steps

    · Products which are more complex to characterize due to low levels/lack of expression in easily available lysates or problems experienced in searching for or developing the correct lysate/cell line

    For these reasons, we release Fast-Track antibodies at a reduced price under the following terms:

    · As these antibodies have not been fully characterized at Abcam, we cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and they are therefore not covered by the full Abpromise guarantee

    · Our experienced Scientific Support Team is happy to offer protocol tips and advice to help improve the results obtained

    · Whenever possible, Abcam will also make available data provided by customers that have bought a Fast-Track antibody, the information provided should be used as a guideline only



    Feedback reward
    We would greatly appreciate your feedback on these antibodies, whether positive or negative. We will award 950 bonus Abpoints to the first researcher who sends us positive feedback (including an image and details of materials and methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with 950 bonus Abpoints. Other useful positive and negative feedback will be rewarded with the standard Abpoints for submitting an Abreview (please refer to Abreviews terms and conditions for more detailed information). All awards are subject to approval by Abcam scientists and provision of suitable data. Please submit feedback to https://mail.google.com/mail/?view=cm&fs=1&tf=1&to=fast-track@abcam.com

    Abpoints Rewards: https://www.abcam.com/index.html?pageconfig=loyalty_rewards_list


    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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    Answer

    Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    As you wish to test the product in an untested application, you may be eligible for our AbTrial program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link:

    www.abcam.com/abtrial

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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