Overview

  • Product name

    Anti-LOK antibody - N-terminal
    See all LOK primary antibodies
  • Description

    Rabbit polyclonal to LOK - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment within Human LOK (N terminal). The exact sequence is proprietary.
    Database link: O94804

  • Positive control

    • WB: HeLa whole cell extract; A431 whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: A431 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 112 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-LOK antibody - N-terminal (ab228802) at 1/2000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : LOK Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 112 kDa

  • All lanes : Anti-LOK antibody - N-terminal (ab228802) at 1/2000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : LOK-knockout HeLa whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 112 kDa



    5% SDS-PAGE gel.

  • Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for LOK (green) using ab228802 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded human breast cancer tissue stained for LOK using ab228802 at 1/250 dilution in immunohistochemical analysis.

  • Anti-LOK antibody - N-terminal (ab228802) at 1/2000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 112 kDa



    5% SDS-PAGE gel.

References

ab228802 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228802.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up