Anti-LONP1/Lon antibody (ab224316)
Key features and details
- Rabbit polyclonal to LONP1/Lon
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-LONP1/Lon antibody
See all LONP1/Lon primary antibodies -
Description
Rabbit polyclonal to LONP1/Lon -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human LONP1/Lon aa 350-550.
Database link: P36776 -
Positive control
- WB: NIH/3T3, NBT-II, PC-12 and SK-MEL-30 cell lysate. ICC/IF: U-251 MG cells. IHC-P: Human duodenum, Fallopian tube and tonsil tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 106 kDa.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 106 kDa. |
Target
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Function
ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner. May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters. Endogenous substrates include mitochondrial steroidogenic acute regulatory (StAR) protein. -
Tissue specificity
Duodenum, heart, lung and liver, but not thymus. -
Involvement in disease
CODAS syndrome -
Sequence similarities
Belongs to the peptidase S16 family.
Contains 1 Lon domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 510796 Cow
- Entrez Gene: 9361 Human
- Entrez Gene: 74142 Mouse
- Entrez Gene: 170916 Rat
- Omim: 605490 Human
- SwissProt: Q59HJ6 Cow
- SwissProt: P36776 Human
- SwissProt: Q8CGK3 Mouse
see all -
Alternative names
- hLON antibody
- hLON ATP dependent protease antibody
- LON antibody
see all
Images
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Paraffin embedded human duodenum tissue stained for LONP1/Lon with ab224316 (1/500 dilution) in immunohistochemical analysis.
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Anti-LONP1/Lon antibody (ab224316) at 1/100 dilution + SK-MEL-30 cell lysate
Predicted band size: 106 kDa -
Paraffin embedded human tonsil tissue stained for LONP1/Lon with ab224316 (1/500 dilution) in immunohistochemical analysis.
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PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for LONP1/Lon (green) using ab224316 (4 µg/ml) in ICC/IF.
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Paraffin embedded human fallopian tissue stained for LONP1/Lon with ab224316 (1/500 dilution) in immunohistochemical analysis.
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All lanes : Anti-LONP1/Lon antibody (ab224316) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Predicted band size: 106 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224316 has been referenced in 1 publication.
- Maneix L et al. The Mitochondrial Protease LonP1 Promotes Proteasome Inhibitor Resistance in Multiple Myeloma. Cancers (Basel) 13:N/A (2021). PubMed: 33671345