Overview

  • Product name

    Anti-Loquacious antibody
  • Description

    Rabbit polyclonal to Loquacious
  • Host species

    Rabbit
  • Specificity

    ab24237 specifically recognises recombinant Loquacious fragment protein. However, ab24237 does not appear to detect endogenous Loquacious in Drosophila embryo lysate, which may be due to low expression in this lysate.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Drosophila melanogaster
  • Immunogen

    Recombinant fragment corresponding to Drosophila melanogaster Loquacious.

  • Positive control

    • This antibody gave a positive signal in the following recombinant protein: Loquacious recombinant (immunizing) fragment protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.

Target

  • Relevance

    Loquacious is a Drosophila double-stranded RNA binding protein that is required for efficient microRNA-mediated gene silencing by Dicer-1. microRNAs (miRNAs) are single-stranded, 21- to 23-nucleotide cellular RNAs that control the expression of cognate target genes.
  • Cellular localization

    Nuclear
  • Database links

    • Alternative names

      • CG6866 antibody
      • loqs antibody
      • R3D1 antibody
      • R3D1 L antibody
      • R3D1 S antibody
      • TRBP antibody
      see all

    Images

    • All lanes : Anti-Loquacious antibody (ab24237) at 1 µg/ml

      Lane 1 : Loquacious recombinant fragment protein
      Lane 2 : Drosophila embryo lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

      Predicted band size: 45 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?



      ab24237 specifically recognises the immunizing Loquacious recombinant fragment protein at 35 kDa (lane1). However, endogenous Loquacious was not detected in Drosophila embryo lysate at 45 kDa (lane2). This may be due to low expression of Loquacious in this lysate.

    References

    ab24237 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer


    I found the information about Loquacious on SwissProt : http://www.uniprot.org/uniprot/?query=Loquacious+AND+organism%3A%22Drosophila+melanogaster+%5B7227%5D%22&sort=score

    Please note that the links to each isoform are also on the product datasheet of ab24237 :
    isoform A : http://www.uniprot.org/uniprot/Q8IP72
    isoform B : http://www.uniprot.org/uniprot/Q9VJY9
    isoform C : http://www.uniprot.org/uniprot/Q4TZM6

    Read More

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