Key features and details
- Rabbit polyclonal to Loquacious
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Product nameAnti-Loquacious antibody
DescriptionRabbit polyclonal to Loquacious
Specificityab24237 specifically recognises recombinant Loquacious fragment protein. However, ab24237 does not appear to detect endogenous Loquacious in Drosophila embryo lysate, which may be due to low expression in this lysate.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Drosophila melanogaster
Recombinant fragment corresponding to Drosophila melanogaster Loquacious.
- This antibody gave a positive signal in the following recombinant protein: Loquacious recombinant (immunizing) fragment protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab24237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.|
RelevanceLoquacious is a Drosophila double-stranded RNA binding protein that is required for efficient microRNA-mediated gene silencing by Dicer-1. microRNAs (miRNAs) are single-stranded, 21- to 23-nucleotide cellular RNAs that control the expression of cognate target genes.
- CG6866 antibody
- loqs antibody
- R3D1 antibody
All lanes : Anti-Loquacious antibody (ab24237) at 1 µg/ml
Lane 1 : Loquacious recombinant fragment protein
Lane 2 : Drosophila embryo lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
ab24237 specifically recognises the immunizing Loquacious recombinant fragment protein at 35 kDa (lane1). However, endogenous Loquacious was not detected in Drosophila embryo lysate at 45 kDa (lane2). This may be due to low expression of Loquacious in this lysate.
ab24237 has not yet been referenced specifically in any publications.