Recombinant
RabMAb

Recombinant Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (ab240345)

Overview

  • Product name

    Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free
    See all Loricrin primary antibodies
  • Description

    Rabbit monoclonal [EPR7149(2)] to Loricrin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Loricrin aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P23490

  • General notes

    Ab240345 is the carrier-free version of ab198994. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240345 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Major keratinocyte cell envelope protein.
  • Involvement in disease

    Defects in LOR are a cause of progressive symmetric erythrokeratodermia (PSEK) [MIM:133200]. Erythrokeratodermas are a group of disorders characterized by widespread erythematous plaques, either stationary or migratory, associated with features that include palmoplantar keratoderma. PSEK is characterized by erythematous and hyperkeratotic plaques.
    Defects in LOR are the cause of Vohwinkel syndrome with ichthyosis (VSI) [MIM:604117]; also known as loricrin keratoderma (LK) or mutilating keratoderma with ichthyosis. VSI is an ichthyotic variant of Vohwinkel syndrome (VS) characterized by progressive symmetric erythrokeratoderma or congenital ichthyosiform erythroderma born as a collodion baby. Common clinical features include hyperkeratosis of the palms and soles with digital constriction.
  • Post-translational
    modifications

    Substrate of transglutaminases. Some glutamines and lysines are cross-linked to other loricrin molecules and to SPRRs proteins.
    Contains inter- or intramolecular disulfide-bonds.
  • Cellular localization

    Cytoplasm. Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • LOR antibody
    • LOR protein antibody
    • LORI_HUMAN antibody
    • Loricrin antibody
    • LRN antibody
    • MGC111513 antibody
    • OTTHUMP00000015823 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of A431 cell line labeling Loricrin with ab198994 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100. Alexa Fluor® 488 Goat anti-Rabbit ab150077 used as the secondary at a 1/1000 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb) at a 1/1000 dilution and ab150120 Alexa Fluor® 594 Goat anti-Mouse secondary at a 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198994).

  • Immunohistochemical analysis of paraffin-embedded rat skin tissue sections labeling Loricrin with ab198994 at a 1/500 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198994).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human uterus tissue sections labeling Loricrin with ab198994 at a 1/500 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin. Used as a negative tissue control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198994).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue sections labeling Loricrin with ab198994 at a 1/500 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198994).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab240345 has not yet been referenced specifically in any publications.

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