Product nameLow Fluorescence Western Membrane (PVDF)
See all Western blot membrane reagents
Tested applicationsSuitable for: WBmore details
Pre-cut membranes (10 sheets, 7 x 9 cm), compatible with gels 10 x 10 cm and smaller. Note: PVDF membranes must be activated prior to use. Membrane activation protocol: 1. Pre-wet the membrane in methanol for a few moments until the membrane is completely wet. 2. Transfer the wet membrane from methanol to purified water and incubate on a rocker or shaker for at least 5 minutes. Make sure that the membrane is fully immersed in water and does not float on the water’s surface. 3. Transfer the membrane from water to transfer buffer, and incubate for 5 minutes.
- Prestained Protein Ladder - Mid-range molecular weight (10-175 kDa) (ab115832)
- Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (ab116027)
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (ab116029)
- TEO-Tricine Precast Gels - RunBlue™ (4-20%, 12-well, 8x10cm) (ab119209)
- InstantBlue® Coomassie Protein Stain (ab119211)
- Bradford Reagent (ab119216)
- ECL Substrate Kit (High Sensitivity) (ab133406)
Our Abpromise guarantee covers the use of ab133411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab133411 has been referenced in 3 publications.
- Belgacem H et al. Lactobacillus plantarum MON03 counteracts zearalenone génotoxicty in mice: Chromosome aberrations, micronuclei, DNA fragmentation and apoptotique gene expression. Mutat Res Genet Toxicol Environ Mutagen 840:11-19 (2019). PubMed: 30857728
- Oppermann H et al. D,L-Methadone does not improve radio- and chemotherapy in glioblastoma in vitro. Cancer Chemother Pharmacol 83:1017-1024 (2019). PubMed: 30888463
- Jebali R et al. Lactobacillus plantarum alleviate aflatoxins (B1 and M1) induced disturbances in the intestinal genes expression and DNA fragmentation in mice. Toxicon 146:13-23 (2018). WB . PubMed: 29574215