Anti-LOX 1 antibody (ab60178)

Rabbit polyclonal LOX 1 antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Human, Pig. Cited in 26 publication(s). Independently reviewed in 3 review(s).

Overview

  • Product name

  • Description

    Rabbit polyclonal to LOX 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide near the N-terminus of human Lox-1

  • Positive control

    • Jurkat whole cell lysate (ab7899)

Properties

Applications

Our Abpromise guarantee covers the use of ab60178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 31 kDa.Can be blocked with LOX 1 peptide (ab183565). Proform detected at 50 kDa, mature form detected at 31 kDa.

Target

  • Function

    Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
  • Tissue specificity

    Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.
  • Involvement in disease

    Note=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
    Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Domain

    The cytoplasmic region is required for subcellular sorting on the cell surface.
    The C-type lectin domain mediates the recognition and binding of oxLDL.
  • Post-translational
    modifications

    The intrachain disulfide-bonds prevent N-glycosylation at some sites.
    N-glycosylated.
  • Cellular localization

    Cell membrane. Secreted. A secreted form also exists.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-type lectin domain family 8 member A antibody
    • CLEC8A antibody
    • hLOX 1 antibody
    • hLOX-1 antibody
    • Lectin like oxidized LDL receptor 1 antibody
    • Lectin like oxLDL receptor 1 antibody
    • Lectin type oxidized LDL receptor 1 antibody
    • Lectin-like oxidized LDL receptor 1 antibody
    • Lectin-like oxLDL receptor 1 antibody
    • Lectin-type oxidized LDL receptor 1 antibody
    • low density lipoprotein oxidized, receptor 1 antibody
    • LOX-1 antibody
    • LOXIN antibody
    • Olr1 antibody
    • OLR1_HUMAN antibody
    • Ox LDL receptor 1 antibody
    • Ox-LDL receptor 1 antibody
    • Oxidised low density lipoprotein (lectin like) receptor 1 antibody
    • Oxidized low density lipoprotein receptor 1 antibody
    • Oxidized low density lipoprotein receptor 1 soluble form antibody
    • Oxidized low-density lipoprotein receptor 1 antibody
    • OxLDL receptor 1 antibody
    • SCARE1 antibody
    • Scavenger receptor class E, member 1 antibody
    • SLOX1 antibody
    • soluble form antibody
    • SR-EI antibody
    see all

Images

  • All lanes : Anti-LOX 1 antibody (ab60178) at 0.5 µg/ml

    All lanes : Jurkat cell lysate

    Predicted band size: 31 kDa
    Observed band size: 31,50 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab60178 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60178, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab60178 staining LOX 1 in mouse heart and carotid artery tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% BSA for 1 hour at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody. Counter stained with DAPI.

    See Abreview

References

This product has been referenced in:

  • Pucci S  et al. Pro-oncogenic action of LOX-1 and its splice variant LOX-1?4 in breast cancer phenotypes. Cell Death Dis 10:53 (2019). Read more (PubMed: 30718451) »
  • Hein TW  et al. Requisite roles of LOX-1, JNK, and arginase in diabetes-induced endothelial vasodilator dysfunction of porcine coronary arterioles. J Mol Cell Cardiol 131:82-90 (2019). Read more (PubMed: 31015037) »
See all 34 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for your enquiry regarding ab60178.

I regret to inform you that we do not have blocking peptide available for this particular antibody. ab60178 has been tested on ICC/IF (immunocytochemistry) but not on formalin-fixed paraffin embedded tissue section (IHC-P). At this stage we do not know how formalin fixation would affect the epitope.

I would suggest running a good positive control i.e. human or mouse small intestine vessel section fixed with non formalin-based fixatives such as ethanol, methanol or acetone. It would be also worth running a no primary - only secondary control to see if the unexpected staining patter could be due to non-specific signal due to the secondary antibody.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart and carotid artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Retrieval sol. H-3300 Vector lab
Permeabilization
No
Specification
heart and carotid artery
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
formalin 10% buffered neutral

Abcam user community

Verified customer

Submitted Oct 21 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Rat Tissue lysate - whole (Thoracic Aorta Tissue Homogenate)
Specification
Thoracic Aorta Tissue Homogenate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 29 2014

Answer

Thank you for your call.

Unfortunately, I have to confirm that the antibodies we have in our catalog are unlikely to definitively not react with pig.

The whole protein shows ca 70% homology between human and pig. Therefore we would not expect a good cross reaction but we also would not eclude all cross reaction.

I checked the single immunogens of our Anti-LOX 1 antibodies and they show homologies between 70% and 73%.

I am sorry to confirm that I cannot recommend any of these antibodies for your experiments.

Read More

Answer

Thank you for contacting us.

I agree, the blurry banding above the proform is likely caused by glycosylations and that the other major band at 31 kDa is the mature form of LOX 1. According to the following reference, the soluble form of LOX1 willrun at a molecular weight of 35 kDa, which would be consistent with the band you observe above the mature form:

http://www.cmj.org/periodical/PDF/20073560275380.pdf

The lower band could represent isoform 2 of LOX 1, which has a predicted molecular weight of 20 kDa according to the UniProt database:

http://www.uniprot.org/uniprot/P78380

I hope this helps, please let me know if you need any additional information or assistance.

Read More

Answer

Thank you for your enquiry. Here are answers to your questions: Q1: Can I have the URL for the EasyLink informational page? A1: https://www.abcam.com/easylink Q2: What is the email address of the person responsible for bulk order requests at Abcam? A2: Please email mailto:sales@abcam.com, with attention to Andrea. In your email please provide the product number, quantity (10 vials or more for a bulk order), what conjugate you would like linked to the antibody and any other pertinent information. I hope this is helpful. Please contact me again if you have any further questions.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (human embryonic kidney cells (293T))
Loading amount
20 µg
Specification
human embryonic kidney cells (293T)
Treatment
transfected with 300ng of plasmid expressing porcine LOX-1 cDNA (NM 213805)
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 19 2010

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