Key features and details
- Rabbit polyclonal to LOX 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab60178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 31 kDa.Can be blocked with LOX 1 peptide (ab183565). Proform detected at 50 kDa, mature form detected at 31 kDa.|
FunctionReceptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
Tissue specificityExpressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.
Involvement in diseaseNote=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not.
Sequence similaritiesContains 1 C-type lectin domain.
DomainThe cytoplasmic region is required for subcellular sorting on the cell surface.
The C-type lectin domain mediates the recognition and binding of oxLDL.
modificationsThe intrachain disulfide-bonds prevent N-glycosylation at some sites.
Cellular localizationCell membrane. Secreted. A secreted form also exists.
- Information by UniProt
- C-type lectin domain family 8 member A antibody
- CLEC8A antibody
- hLOX 1 antibody
All lanes : Anti-LOX 1 antibody (ab60178) at 0.5 µg/ml
All lanes : Jurkat cell lysate
Predicted band size: 31 kDa
Observed band size: 31,50 kDa why is the actual band size different from the predicted?
ab60178 has been referenced in 41 publications.
- Niu YY et al. Elevated intracellular copper contributes a unique role to kidney fibrosis by lysyl oxidase mediated matrix crosslinking. Cell Death Dis 11:211 (2020). PubMed: 32235836
- Qian W et al. Astragaloside IV protects endothelial progenitor cells from the damage of ox-LDL via the LOX-1/NLRP3 inflammasome pathway. Drug Des Devel Ther 13:2579-2589 (2019). PubMed: 31440038
- Pucci S et al. Pro-oncogenic action of LOX-1 and its splice variant LOX-1?4 in breast cancer phenotypes. Cell Death Dis 10:53 (2019). PubMed: 30718451
- Hein TW et al. Requisite roles of LOX-1, JNK, and arginase in diabetes-induced endothelial vasodilator dysfunction of porcine coronary arterioles. J Mol Cell Cardiol 131:82-90 (2019). PubMed: 31015037
- Lu J et al. Active polypeptides from Hirudo inhibit endothelial cell inflammation and macrophage foam cell formation by regulating the LOX-1/LXR-a/ABCA1 pathway. Biomed Pharmacother 115:108840 (2019). PubMed: 31048189