Overview

  • Product name

  • Description

    Goat polyclonal to LOX
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide corresponding to Human LOX aa 247-260 (internal sequence) (Cysteine residue). NP_002308.2; NP_001171573.1.
    Sequence:

    C-STAYRADVRDYDHR


    Database link: P28300

  • Positive control

    • WB: Human placenta and heart lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab223488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30-33 kDa (predicted molecular weight: 46.9 kDa).

Target

  • Function

    Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression.
  • Tissue specificity

    Heart, placenta, skeletal muscle, kidney, lung and pancreas.
  • Involvement in disease

    Defects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100].
  • Sequence similarities

    Belongs to the lysyl oxidase family.
  • Post-translational
    modifications

    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • lox antibody
    • LYOX antibody
    • LYOX_HUMAN antibody
    • Lysyl oxidase antibody
    • MGC105112 antibody
    • Protein lysine 6 oxidase antibody
    • Protein-lysine 6-oxidase antibody
    see all

Images

  • All lanes : Anti-LOX antibody (ab223488) at 0.5 µg/ml

    Lane 1 : Human heart lysate in RIPA buffer
    Lane 2 : Human placenta lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 46.9 kDa
    Observed band size: 30-33 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223488 has not yet been referenced specifically in any publications.

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