Key features and details
- Goat polyclonal to LOX
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LOX antibody
See all LOX primary antibodies
DescriptionGoat polyclonal to LOX
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig
- WB: Human placenta and heart lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab223488 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30-33 kDa (predicted molecular weight: 46.9 kDa).
1 hour primary incubation is recommended for this product.
FunctionResponsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression.
Tissue specificityHeart, placenta, skeletal muscle, kidney, lung and pancreas.
Involvement in diseaseDefects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100].
Sequence similaritiesBelongs to the lysyl oxidase family.
modificationsThe lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- lox antibody
- LYOX antibody
- LYOX_HUMAN antibody
All lanes : Anti-LOX antibody (ab223488) at 0.5 µg/ml
Lane 1 : Human heart lysate in RIPA buffer
Lane 2 : Human placenta lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 46.9 kDa
Observed band size: 30-33 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab223488 has not yet been referenced specifically in any publications.