Recombinant

Recombinant Anti-LOX antibody [EPR21203] (ab221936)

Rabbit monoclonal LOX antibody [EPR21203]. Validated in IHC and tested in Mouse, Human.

Overview

  • Product name

    Anti-LOX antibody [EPR21203]
    See all LOX primary antibodies
  • Description

    Rabbit monoclonal [EPR21203] to LOX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Mouse LOX.
    Database link: P28301

  • Positive control

    • IHC-P: FFPE human and mouse skin tissue sections
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Properties

Applications

Our Abpromise guarantee covers the use of ab221936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.05 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression.
  • Tissue specificity

    Heart, placenta, skeletal muscle, kidney, lung and pancreas.
  • Involvement in disease

    Defects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100].
  • Sequence similarities

    Belongs to the lysyl oxidase family.
  • Post-translational
    modifications

    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • lox antibody
    • LYOX antibody
    • LYOX_HUMAN antibody
    • Lysyl oxidase antibody
    • MGC105112 antibody
    • Protein lysine 6 oxidase antibody
    • Protein-lysine 6-oxidase antibody
    see all

Images

  • IHC image of LOX staining in mouse normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab221936, 0.5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of LOX staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab221936, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab221936 has not yet been referenced specifically in any publications.

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