Recombinant Anti-LOX antibody [EPR4025] (ab174316)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4025] to LOX
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-LOX antibody [EPR4025]
See all LOX primary antibodies -
Description
Rabbit monoclonal [EPR4025] to LOX -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, (HeLa (Human cervix adenocarcinoma epithelial cell) treated with 0.5 mM DFO for 24 hours whole cell lysates ) Jurkat and WI-38 cell lysates. Human muscle tissue. HeLa cells. Immunoprecipitation pellet from WI-38 cells lysate. Immunocytochemistry: Human chondrocytes
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4025 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
For unpurified, use 1/1000 - 1/2000. |
IHC-P |
1/900. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified, use 1/300. |
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ICC/IF | (1) |
1/300.
For unpurified, use 1/100. |
IP |
1/10 - 1/100.
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Flow Cyt (Intra) |
1/100.
For unpurified, use 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa. For unpurified, use 1/1000 - 1/2000. |
IHC-P
1/900. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified, use 1/300. |
ICC/IF
1/300. For unpurified, use 1/100. |
IP
1/10 - 1/100. |
Flow Cyt (Intra)
1/100. For unpurified, use 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. -
Tissue specificity
Heart, placenta, skeletal muscle, kidney, lung and pancreas. -
Involvement in disease
Defects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. -
Sequence similarities
Belongs to the lysyl oxidase family. -
Post-translational
modificationsThe lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 4015 Human
- Entrez Gene: 16948 Mouse
- Entrez Gene: 24914 Rat
- Omim: 153455 Human
- SwissProt: P28300 Human
- SwissProt: P28301 Mouse
- SwissProt: P16636 Rat
- Unigene: 102267 Human
see all -
Alternative names
- lox antibody
- LYOX antibody
- LYOX_HUMAN antibody
see all
Images
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Flow Cytometry (Intracellular) analysis of ab174316. HeLa (Human cervix adenocarcinoma epithelial cell) cells were fixed in 4% paraformaldehyde and permeabilized with 90% methanol. ab174316 was used at 1:100 dilution (1ug) (Red). Secondary antibody Goat anti rabbit IgG (Alexa Fluor® 647, ab150083) at 1:5000 dilution. Isotype control Rabbit monoclonal IgG (ab172730) (Black). Unlabelled control, cells without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-LOX antibody [EPR4025] (ab174316) at 1/1000 dilution
Lane 1 : Wild-type HeLa Vehicle Control DFO (0 mM, 24 h) cell lysate
Lane 2 : Wild-type HeLa Treated DFO (0.5 mM, 24 h) cell lysate
Lane 3 : LOX knockout HeLa Vehicle Control DFO (0 mM, 24 h) cell lysate
Lane 4 : LOX knockout HeLa Treated DFO (0.5 mM, 24 h) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-LOX antibody [EPR4025] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab174316 was shown to bind specifically to LOX. A band was observed at 52 kDa in wild-type HeLa cell lysates with no signal observed at this size in Lox knockout cell line ab261801 (knockout cell lysate ab256981). To generate this image, wild-type and Lox knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes :
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 0.5 mM DFO for 24 hours whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 7 secondsPrimary antibody dilution:
ab174316 Anti-LOX antibody 1:5000 dilution (0.1mg/ml)
ab179483 Anti-HIF-1 alpha antibody 1:500 dilution (0.3mg/ml)
Blocking buffer / Dilution buffer and concentration:
5% NFDM/TBST
LOX and HIF-1 alpha could be induced under hypoxia condition (PMID: 23545606, PMID: 30226558).
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All lanes : Anti-LOX antibody [EPR4025] (ab174316) at 1/3700 dilution (purified)
Lane 1 : WI-38 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Mouse brain
Lane 4 : Rat brain
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human kidney with purified ab174316 at a working dilution of 1 in 900. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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Immunofluorescence staining of Jurkat cells with purified ab174316 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.
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Immunocytochemistry/ Immunofluorescence analysis of formaldehyde-fixed Triton X-100 permeabilized human chondrocytes staining with ab174316 at 1/100 dilution in PBS with 0.1% triton x100. Secondary antibody was Alexa Fluor® 488 Goat Anti-Rabbit at 1/2000 dilution. Samples were incubated with the primary antibody for 30 minutes at 37°C. Blocking was with 0.1% BSA for 30 minutes at 37°C (ref: PMC7204390)
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Western blot analysis on immunoprecipitation pellet from (Lane 1) WI-38 (Human fetal lung fibroblast cell line) cells lysate or (Lane 2) 1X PBS (negative control) using unpurified ab174316 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab174316 has been referenced in 37 publications.
- Alfano M et al. Lysyl-Oxidase Dependent Extracellular Matrix Stiffness in Hodgkin Lymphomas: Mechanical and Topographical Evidence. Cancers (Basel) 14:N/A (2022). PubMed: 35008423
- S Ramos K et al. Degree of Fibrosis in Human Atrial Tissue Is Not the Hallmark Driving AF. Cells 11:N/A (2022). PubMed: 35159236
- Tsang KM et al. Copper-Binding Domain Variation in a Novel Murine Lysyl Oxidase Model Produces Structurally Inferior Aortic Elastic Fibers Whose Failure Is Modified by Age, Sex, and Blood Pressure. Int J Mol Sci 23:N/A (2022). PubMed: 35743192
- Manasieva V et al. Semicarbazide-Sensitive Amine Oxidase (SSAO) and Lysyl Oxidase (LOX) Association in Rat Aortic Vascular Smooth Muscle Cells. Biomolecules 12:N/A (2022). PubMed: 36358914
- Khajvand-Abedini M et al. The Restoring Effect of Human Umbilical Cord-Derived Mesenchymal Cell-Conditioned Medium (hMSC-CM) against Carbon Tetrachloride-Induced Pulmonary Fibrosis in Male Wistar Rats. Int J Inflam 2022:7179766 (2022). PubMed: 36588784