Recombinant
RabMAb

Recombinant Anti-LOX antibody [EPR4025] (ab174316)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4025] to LOX
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LOX antibody [EPR4025]
    See all LOX primary antibodies

  • Description

    Rabbit monoclonal [EPR4025] to LOX

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LOX aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P28300

  • Positive control

    • HeLa, Jurkat and WI-38 cell lysates. Human muscle tissue. HeLa cells. Immunoprecipitation pellet from WI-38 cells lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab174316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.

For unpurified, use 1/1000 - 1/2000.
IHC-P 1/900. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified, use 1/300.

IHC antigen retrieval protocols.
ICC/IF 1/300.

For unpurified, use 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/90.

For unpurified, use 1/30. 
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression.

  • Tissue specificity

    Heart, placenta, skeletal muscle, kidney, lung and pancreas.

  • Involvement in disease

    Defects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100].

  • Sequence similarities

    Belongs to the lysyl oxidase family.

  • Post-translational
    modifications

    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • lox antibody
    • LYOX antibody
    • LYOX_HUMAN antibody
    • Lysyl oxidase antibody
    • MGC105112 antibody
    • Protein lysine 6 oxidase antibody
    • Protein-lysine 6-oxidase antibody
    see all

Images

  • Western blot - Anti-LOX antibody [EPR4025] (ab174316)
    Western blot - Anti-LOX antibody [EPR4025] (ab174316)
    All lanes : Anti-LOX antibody [EPR4025] (ab174316) at 1/3700 dilution (purified)

    Lane 1 : WI-38 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Mouse brain
    Lane 4 : Rat brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (ab174316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (ab174316)

    Immunohistochemical staining of paraffin embedded human kidney with purified ab174316 at a working dilution of 1 in 900. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

  • Immunocytochemistry/ Immunofluorescence - Anti-LOX antibody [EPR4025] (ab174316)
    Immunocytochemistry/ Immunofluorescence - Anti-LOX antibody [EPR4025] (ab174316)

    Immunofluorescence staining of Jurkat cells with purified ab174316 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.

  • Flow Cytometry - Anti-LOX antibody [EPR4025] (ab174316)
    Flow Cytometry - Anti-LOX antibody [EPR4025] (ab174316)

    Overlay histogram showing Jurkat cells fixed in 2% PFA and stained with purified ab174316 at a dilution of 1 in 90 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

  • Western blot - Anti-LOX antibody [EPR4025] (ab174316)
    Western blot - Anti-LOX antibody [EPR4025] (ab174316)
    All lanes : Anti-LOX antibody [EPR4025] (ab174316) at 1/1100 dilution (unpurified)

    Lane 1 : WI-38 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Mouse brain
    Lane 4 : Rat brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-LOX antibody [EPR4025] (ab174316)
    Western blot - Anti-LOX antibody [EPR4025] (ab174316)
    All lanes : Anti-LOX antibody [EPR4025] (ab174316) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysates
    Lane 2 : Jurkat cell lysates
    Lane 3 : WI-38 cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (ab174316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (ab174316)

    Immunohistochemical analysis of paraffin-embedded human muscle tissue labeling LOX with unpurified ab174316 at a 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (ab174316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (ab174316)

    Immunohistochemical staining of paraffin embedded human kidney with unpurified ab174316 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

  • Immunocytochemistry/ Immunofluorescence - Anti-LOX antibody [EPR4025] (ab174316)
    Immunocytochemistry/ Immunofluorescence - Anti-LOX antibody [EPR4025] (ab174316)

    Immunofluorescence staining of Jurkat cells with unpurified ab174316 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.

  • Immunocytochemistry/ Immunofluorescence - Anti-LOX antibody [EPR4025] (ab174316)
    Immunocytochemistry/ Immunofluorescence - Anti-LOX antibody [EPR4025] (ab174316)

    Immunofluorescence analysis of HeLa cells labeling LOX with unpurified ab174316 at a 1/50 dilution.

  • Flow Cytometry - Anti-LOX antibody [EPR4025] (ab174316)
    Flow Cytometry - Anti-LOX antibody [EPR4025] (ab174316)

    Flow cytometric analysis of permeabilized Jurkat cells using unpurified ab174316 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Immunoprecipitation - Anti-LOX antibody [EPR4025] (ab174316)
    Immunoprecipitation - Anti-LOX antibody [EPR4025] (ab174316)

    Western blot analysis on immunoprecipitation pellet from (Lane 1) WI-38 (Human fetal lung fibroblast cell line) cells lysate or (Lane 2) 1X PBS (negative control) using unpurified ab174316 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

References (16)

Publishing research using ab174316? Please let us know so that we can cite the reference in this datasheet.

ab174316 has been referenced in 16 publications.

  • Despotovic SZ  et al. Altered organization of collagen fibers in the uninvolved human colon mucosa 10?cm and 20?cm away from the malignant tumor. Sci Rep 10:6359 (2020). PubMed: 32286443
  • Klepfish M  et al. LOXL2 Inhibition Paves the Way for Macrophage-Mediated Collagen Degradation in Liver Fibrosis. Front Immunol 11:480 (2020). PubMed: 32296422
  • Sharma P  et al. Biallelic HEPHL1 variants impair ferroxidase activity and cause an abnormal hair phenotype. PLoS Genet 15:e1008143 (2019). PubMed: 31125343
  • Natarajan S  et al. Collagen Remodeling in the Hypoxic Tumor-Mesothelial Niche Promotes Ovarian Cancer Metastasis. Cancer Res 79:2271-2284 (2019). PubMed: 30862717
  • Mu J  et al. BRD4 promotes tumor progression and NF-?B/CCL2-dependent tumor-associated macrophage recruitment in GIST. Cell Death Dis 10:935 (2019). PubMed: 31819043
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

IHC - Wholemount abreview for Anti-LOX antibody [EPR4025]

 Average
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Zebrafish Embryo (3 days post fertilization)
Specification
3 days post fertilization
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Abcam user community

Verified customer

Submitted May 22 2017

Western blot abreview for Anti-LOX antibody [EPR4025]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole embryo)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
150 µg
Specification
whole embryo
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted Apr 25 2017

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