Recombinant Anti-LOX antibody [EPR4025] (ab174316)

Blocking buffer: 5% NFDM/TBST

Dilution buffer: 5% NFDM/TBST

Immunohistochemical staining of paraffin embedded human kidney with purified ab174316 at a working dilution of 1 in 900. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunofluorescence staining of Jurkat cells with purified ab174316 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.

Overlay histogram showingJurkat cells fixed in 2% PFA and stained with purified ab174316 at a dilution of 1 in 90 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control. 

Blocking buffer: 5% NFDM/TBST

Dilution buffer: 5% NFDM/TBST

Immunohistochemical analysis of paraffin-embedded human muscle tissue labeling LOX with unpurified ab174316 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemical staining of paraffin embedded human kidney with unpurified ab174316 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunofluorescence staining of Jurkat cells with unpurified ab174316 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.

Immunofluorescence analysis of HeLa cells labeling LOX with unpurified ab174316 at a 1/50 dilution.

Intracellular flow cytometric analysis of permeabilized Jurkat cells using unpurified ab174316 at a 1/10 dilution (red) or a rabbit IgG (negative) (green). 

Western blot analysis on immunoprecipitation pellet from (Lane 1) WI-38 (Human fetal lung fibroblast cell line) cells lysate or (Lane 2) 1X PBS (negative control) using unpurified ab174316 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.