Name: Anti-LOX antibody [EPR4025]
Brand: RabMAb
SKU: ab174316
Rating: 3 (2 reviews)

Product name

Anti-LOX antibody [EPR4025]
See all LOX primary antibodies
Description

Rabbit monoclonal [EPR4025] to LOX
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LOX aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P28300
Positive control
- HeLa, Jurkat and WI-38 cell lysates. Human muscle tissue. HeLa cells. Immunoprecipitation pellet from WI-38 cells lysate.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4025
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Immunizing Peptide (Blocking)
- LOX peptide (ab207571)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

Our Abpromise guarantee covers the use of ab174316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 47 kDa.Can be blocked with LOX peptide (ab207571). For unpurified, use 1/1000 - 1/2000.
IHC-P		1/900. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified, use 1/300. IHC antigen retrieval protocols.
ICC/IF		1/300. For unpurified, use 1/100.
IP		1/10 - 1/100.
Flow Cyt		1/90. For unpurified, use 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Function

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression.
Tissue specificity

Heart, placenta, skeletal muscle, kidney, lung and pancreas.
Involvement in disease

Defects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100].
Sequence similarities

Belongs to the lysyl oxidase family.
Post-translational
modifications

The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
Cellular localization

Secreted > extracellular space.
Target information above from: UniProt accession P28300 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4015 Human
- Entrez Gene: 16948 Mouse
- Entrez Gene: 24914 Rat
- Omim: 153455 Human
- SwissProt: P28300 Human
- SwissProt: P28301 Mouse
- SwissProt: P16636 Rat
- Unigene: 102267 Human
- Unigene: 172 Mouse
- Unigene: 11372 Rat
see all
Alternative names
- lox antibody
- LYOX antibody
- LYOX_HUMAN antibody
- Lysyl oxidase antibody
- MGC105112 antibody
- Protein lysine 6 oxidase antibody
- Protein-lysine 6-oxidase antibody
see all

Western blot - Anti-LOX antibody [EPR4025] (ab174316)

All lanes : Anti-LOX antibody [EPR4025] (ab174316) at 1/3700 dilution (purified)

Lane 1 : WI-38 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Mouse brain
Lane 4 : Rat brain

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (ab174316)

Immunohistochemical staining of paraffin embedded human kidney with purified ab174316 at a working dilution of 1 in 900. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunocytochemistry/ Immunofluorescence - Anti-LOX antibody [EPR4025] (ab174316)

Immunofluorescence staining of Jurkat cells with purified ab174316 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.
Flow Cytometry - Anti-LOX antibody [EPR4025] (ab174316)

Overlay histogram showing Jurkat cells fixed in 2% PFA and stained with purified ab174316 at a dilution of 1 in 90 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.
Western blot - Anti-LOX antibody [EPR4025] (ab174316)

All lanes : Anti-LOX antibody [EPR4025] (ab174316) at 1/1100 dilution (unpurified)

Lane 1 : WI-38 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Mouse brain
Lane 4 : Rat brain

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-LOX antibody [EPR4025] (ab174316)

All lanes : Anti-LOX antibody [EPR4025] (ab174316) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : WI-38 cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (ab174316)

Immunohistochemical analysis of paraffin-embedded human muscle tissue labeling LOX with unpurified ab174316 at a 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (ab174316)

Immunohistochemical staining of paraffin embedded human kidney with unpurified ab174316 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunocytochemistry/ Immunofluorescence - Anti-LOX antibody [EPR4025] (ab174316)

Immunofluorescence staining of Jurkat cells with unpurified ab174316 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.
Immunocytochemistry/ Immunofluorescence - Anti-LOX antibody [EPR4025] (ab174316)

Immunofluorescence analysis of HeLa cells labeling LOX with unpurified ab174316 at a 1/50 dilution.
Flow Cytometry - Anti-LOX antibody [EPR4025] (ab174316)

Flow cytometric analysis of permeabilized Jurkat cells using unpurified ab174316 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunoprecipitation - Anti-LOX antibody [EPR4025] (ab174316)

Western blot analysis on immunoprecipitation pellet from (Lane 1) WI-38 cells lysate or (Lane 2) 1X PBS (negative control) using unpurified ab174316 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Lai H et al. FAK-ERK activation in cell/matrix adhesion induced by the loss of apolipoprotein E stimulates the malignant progression of ovarian cancer. J Exp Clin Cancer Res 37:32 (2018). Read more (PubMed: 29458390) »
Hsiung S et al. Hyperglycemia does not affect tissue repair responses in shear stress-induced atherosclerotic plaques in ApoE-/- mice. Sci Rep 8:7530 (2018). Read more (PubMed: 29760458) »

See all 7 Publications for this product

Publishing research using ab174316? Please let us know so that we can cite the reference in this datasheet.

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1-2 of 2 Abreviews or Q&A

Application

IHC - Wholemount

Sample

Zebrafish Embryo (3 days post fertilization)

Specification

3 days post fertilization

Abcam user community

Verified customer

Submitted May 22 2017

Abcam has not validated the combination of species/application used in this Abreview.

Application

Western blot

Sample

Zebrafish Tissue lysate - whole (whole embryo)

Gel Running Conditions

Reduced Denaturing (10% SDS-PAGE)

Loading amount

150 µg

Specification

whole embryo

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 25 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-LOX antibody [EPR4025] (ab174316)

Overview

Properties

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Applications

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Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

IHC - Wholemount abreview for Anti-LOX antibody [EPR4025]

Western blot abreview for Anti-LOX antibody [EPR4025]