Recombinant
RabMAb

Recombinant Anti-LOX antibody [EPR4025] - Low endotoxin, Azide free (ab219369)

Overview

  • Product name

    Anti-LOX antibody [EPR4025] - Low endotoxin, Azide free
    See all LOX primary antibodies
  • Description

    Rabbit monoclonal [EPR4025] to LOX - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LOX aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P28300

  • Positive control

    • HeLa, Jurkat and WI-38 cell lysates. Human muscle tissue. HeLa cells. Immunoprecipitation pellet from WI-38 cells lysate.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab219369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.Can be blocked with LOX peptide (ab207571).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols IHC antigen retrieval protocols.

ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression.
  • Tissue specificity

    Heart, placenta, skeletal muscle, kidney, lung and pancreas.
  • Involvement in disease

    Defects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100].
  • Sequence similarities

    Belongs to the lysyl oxidase family.
  • Post-translational
    modifications

    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • lox antibody
    • LYOX antibody
    • LYOX_HUMAN antibody
    • Lysyl oxidase antibody
    • MGC105112 antibody
    • Protein lysine 6 oxidase antibody
    • Protein-lysine 6-oxidase antibody
    see all

Images

  • Overlay histogram showing Jurkat cells fixed in 2% PFA and stained with purified ab174316 at a dilution of 1 in 90 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174316).

  • Immunofluorescence staining of Jurkat cells with purified ab174316 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174316).

  • Immunofluorescence staining of Jurkat cells with unpurified ab174316 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 200. The cells were fixed in 4% PFA.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174316).

  • Immunohistochemical staining of paraffin embedded human kidney with purified ab174316 at a working dilution of 1 in 900. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174316).

  • Immunohistochemical staining of paraffin embedded human kidney with unpurified ab174316 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174316).

  • Immunohistochemical analysis of paraffin-embedded human muscle tissue labeling LOX with unpurified ab174316 at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174316).

  • Immunofluorescence analysis of HeLa cells labeling LOX with unpurified ab174316 at a 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174316).

  • Flow cytometric analysis of permeabilized Jurkat cells using unpurified ab174316 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174316).

  • Western blot analysis on immunoprecipitation pellet from (Lane 1) WI-38 cells lysate or (Lane 2) 1X PBS (negative control) using unpurified ab174316 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174316).

References

ab219369 has not yet been referenced specifically in any publications.

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