Product nameLoxistatin Acid (E-64C), cysteine protease inhibitor
DescriptionE-64 (ab141418) derivative. Irreversible cysteine protease inhibitor.
- (+)-(2S,3S)-3-[[(S)-3-methyl-1- [(3-methylbutyl)carbamoyl]butyl]carbamoyl]-2-oxiranecarboxylic acid
Synthetic analog of E-64 (ab141418), a potent, irreversible cysteine protease inhibitor isolated from Aspergillus japonicus. Effective calpain inhibitor, which also inhibits cathepsin B, cathepsin H, and cathepsin L. Inhibits calpain activity in intact platelets.
Chemical name(2S,3S)-3-[[[(1S)-3-Methyl-1-[[(3-methylbutyl)amino]carbonyl]butyl]amino]carbonyl]-2-oxiranecarboxylic acid
Molecular formulaC15H26 N2 O5
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM, in water to 5 mM and in ethanol to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Huang Z et al. Ester and amide derivatives of E64c as inhibitors of platelet calpains. J Med Chem 35:2048-54 (1992). Read more (PubMed: 1597856) »
- Yamamoto D et al. Refined x-ray structure of papain.E-64-c complex at 2.1-A resolution. J Biol Chem 266:14771-7 (1991). Read more (PubMed: 1860874) »
- Inuzuka T et al. Suppressive effect of E-64c on ischemic degradation of cerebral proteins following occlusion of the middle cerebral artery in rats. Brain Res 526:177-9 (1990). Read more (PubMed: 1706637) »
- Barrett AJ et al. L-trans-Epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L. Biochem J 201:189-98 (1982). Read more (PubMed: 7044372) »
- Noda T et al. Effects of cathepsin B, H, and D in pectoral muscle of dystrophic chickens (line 413) of in vivo administration of E-64-c (N-[N-(L-3-transcarboxyoxirane-2-carbonyl)-L-leucyl]-3-methyl-butylamine). J Biochem 90:893-6 (1981). Read more (PubMed: 7309707) »