Overview

  • Product name

  • Description

    Rabbit polyclonal to LOXL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cat
  • Immunogen

    Synthetic peptide: RWRQLIQWEN NGQVYSLLNS GSEYVPAGPQ RSESSSRVLL AGAPQAQQRR , corresponding to a region within N terminal amino acids 38 - 87 of Human LOXL1 (NP_005567)

  • Positive control

    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IP Use a concentration of 5 µg/ml.

Target

  • Relevance

    LOXL1 is a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. LOXL1 is active on elastin and collagen substrates. Genetic variations in LOXL1 are associated with risk of developing exfoliation syndrome (XFS) [MIM:177650]; also called exfoliation glaucoma (XFG). Exfoliation syndrome (XFS) is characterized by accumulation of abnormal microfibrillar deposits that line the aqueous bathed surfaces of the anterior segment of the eye. The prevalence of XFS increases with age, and a number of studies have pointed to a geographical clustering of XFS, although this condition is found worldwide; reported prevalence rates average about 10 to 20% of the general population over age 60.
  • Cellular localization

    Secreted, extracellular space.
  • Database links

  • Alternative names

    • Lysyl oxidase like 1 antibody
    • LOL antibody
    • LOXL antibody
    • Lysyl oxidase homolog 1 antibody
    • Lysyl oxidase like protein 1 antibody
    see all

Images

  • Anti-LOXL1 antibody (ab81488) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 63 kDa

  • LOXL1 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to LOXL1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab81488.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 63kDa; LOXL1

References

This product has been referenced in:

  • Terajima M  et al. Cyclophilin B control of lysine post-translational modifications of skin type I collagen. PLoS Genet 15:e1008196 (2019). Read more (PubMed: 31173582) »
  • Mižíková I  et al. Collagen and elastin cross-linking is altered during aberrant late lung development associated with hyperoxia. Am J Physiol Lung Cell Mol Physiol 308:L1145-58 (2015). Read more (PubMed: 25840994) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEFs)
Loading amount
5 µg
Specification
MEFs
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Raul Peña

Verified customer

Submitted Feb 22 2013

Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
5 µg
Specification
293T
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%

Mr. Raul Peña

Verified customer

Submitted Feb 22 2013

Answer

Thank you for contacting Abcam. As requested please find attached the Certificate of Analysis and MSDS for: ab66339 ab81488 If there is anything else that we can help you with, please do not hesitate to contact us.

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