Overview

  • Product name

  • Description

    Rabbit polyclonal to LOXL2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Xenopus laevis, Pufferfish, Monkey, Zebrafish, Gorilla, Opossum, Common marmoset, Orangutan, Bat, Platypus, Elephant
  • Immunogen

    Synthetic 15 amino acid peptide from N-terminus of Human LOXL2.

  • Positive control

    • Human prostate and Human liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab133383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded Human prostate tissue labelling LOXL2 with ab133383 at 15µg/ml. Followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemical analysis of formalin-fixed, paraffin embedded Human liver tissue labelling LOXL2 with ab133383 at 15µg/ml. Followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab133383 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Human Cell lysate - whole cell (COS7)
Specification
COS7
Treatment
hloxl2-HAtag expression vector overexpression
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Sep 25 2013

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