Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Human Cell lysate - whole cell (COS7)
Specification
COS7
Treatment
hloxl2-HAtag expression vector overexpression
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Other product details
Concentration
4 µg/ml
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Positive control
hLoxl2-HA was detected with anti-HA antibody.
Additional data
Abcam response
Thank you for your valuable feedback and for participating in our Abreview testing discount program. We are sorry to hear that this antibody has not given you good results in western blot using human sample. This antibody has not been tested with this application before, and it is possible that it may not be suitable for use.
Abcam user community
Verified customer
Submitted Sep 25 2013