Overview

  • Product name

  • Description

    Rabbit polyclonal to LOXL2
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Monkey, Gorilla, Bat, Elephant
  • Immunogen

    A synthetic 20 amino acid peptide corresponding to an internal region of Human LOXL2 (NP_002309.1).

  • Positive control

    • Human prostate and liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab140833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate.
  • Sequence similarities

    Belongs to the lysyl oxidase family.
    Contains 4 SRCR domains.
  • Post-translational
    modifications

    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • LOR 2 antibody
    • LOR2 antibody
    • LOX L2 antibody
    • LOXL 2 antibody
    • LOXL2 antibody
    • LOXL2_HUMAN antibody
    • Lysyl oxidase homolog 2 antibody
    • Lysyl oxidase like 2 antibody
    • Lysyl oxidase like protein 2 antibody
    • Lysyl oxidase related 2 antibody
    • Lysyl oxidase related protein 2 antibody
    • Lysyl oxidase related protein WS9 14 antibody
    • Lysyl oxidase-like protein 2 antibody
    • Lysyl oxidase-related protein 2 antibody
    • Lysyl oxidase-related protein WS9-14 antibody
    • WS9 14 antibody
    see all

Images

  • ab140833 staining LOXL2 in mouse tail tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was via the microwave method for 5 minutes. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab140833 staining LOXL2 in Human cartilage tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and permeabilized with 0.2% Trioton X-100 in PBS; antigen retrieval was by heat mediation in a sodium citrate buffer (10mM sodium citrate, 0.05% Tween 20, pH 6.0). Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An undiluted biotin-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed paraffin embedded Human prostate, labelling LOXL2 using ab140833 at 15 μg/ml
  • Immunohistochemical analysis of formalin-fixed paraffin embedded Human liver, labelling LOXL2 using ab140833 at 15 μg/ml

References

ab140833 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Other
Sample
Mouse Tissue sections (mouse knee + leg)
Specification
mouse knee + leg
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 24 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Other - Buffer/Enzyme Used: 10mM Sodium Citrate, 0.05% Tween
Sample
Mouse Tissue sections (Mouse Tail)
Specification
Mouse Tail
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde

Dr. Steffen Rickelt

Verified customer

Submitted Mar 12 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Microwave Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% T ween 20, pH 6.0)
Sample
Human Tissue sections (human cartilage)
Specification
human cartilage
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde

Dr. Steffen Rickelt

Verified customer

Submitted Dec 23 2013

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