Anti-LOXL2 antibody (ab96233)
Key features and details
- Rabbit polyclonal to LOXL2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-LOXL2 antibody
See all LOXL2 primary antibodies -
Description
Rabbit polyclonal to LOXL2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human LOXL2. Recombinant fragment, corresponding to a region within amino acids 454-712 of Human LOXL2.
Database link: Q9Y4K0 -
Positive control
- IHC-P: Mouse ovary tissue and human esophageal cancer tissue. WB: Human samples only: MDA-MB-231 cell line, 293T, A431 and H1299 whole cell lysates, HeLa cell lysate. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (2) |
1/100 - 1/1000.
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WB | (3) |
1/500 - 1/10000. Predicted molecular weight: 87 kDa.
Blocking: 5% non-fat milk in TBST at room temperature 60 minutes. Please ensure to include a positive control in your experiment, examples are included on this datasheet. WB application is validated in human samples only. ab179810 can be used as an alternative product for WB using mouse samples. |
IHC-P | (2) |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/10000. Predicted molecular weight: 87 kDa. Blocking: 5% non-fat milk in TBST at room temperature 60 minutes. Please ensure to include a positive control in your experiment, examples are included on this datasheet. WB application is validated in human samples only. ab179810 can be used as an alternative product for WB using mouse samples. |
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate. -
Sequence similarities
Belongs to the lysyl oxidase family.
Contains 4 SRCR domains. -
Post-translational
modificationsThe lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 532684 Cow
- Entrez Gene: 4017 Human
- Entrez Gene: 94352 Mouse
- Entrez Gene: 290350 Rat
- Omim: 606663 Human
- SwissProt: A6H737 Cow
- SwissProt: Q9Y4K0 Human
- SwissProt: P58022 Mouse
see all -
Alternative names
- LOR 2 antibody
- LOR2 antibody
- LOX L2 antibody
see all
Images
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Immunohistochemical analysis of Paraffin-embedded human esophageal carcinoma tissue labeling LOXL2 with ab96233 at 1/3000 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30min. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Primary antibody incubation: 4°C overnight. Secondary antibody incubation: ABC HRP Kit (Rabbit IgG), 1:200, RT, 30min. Washing condition: PBS, 2 x 5 mins. Chromogen system: DAB.
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All lanes : Anti-LOXL2 antibody (ab96233) at 1/500 dilution (4? overnight)
Lane 1 : WT HeLa cell lysate at 30 µg
Lane 2 : LOXL2 knockout (KO) HeLa cell lysate
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 87 kDa7.5% SDS-PAGE.
alpha Tubulin was used as a loading control and showed equal loading.
Running: 80V, 15min; 140V, 40 min. Transfer: Semi-dry, 18 V, 60 min (NC embrane). Blocking: 5% non-fat milk in TBST, RT, 60 minutes. Washing: 5 ml TBST, 4 x 5 min. ECL exposure.
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Immunohistochemical analysis of paraffin-embedded mouse ovary tissue labeling LOXL2 with ab96233 at 1/500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30min. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Primary antibody incubation: 4°C overnight. Secondary antibody incubation: ABC HRP Kit (Rabbit IgG), 1:200, RT, 30min. Washing condition: PBS, 2 x 5 mins. Chromogen system: DAB.
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All lanes : Anti-LOXL2 antibody (ab96233) at 1/1000 dilution
Lane 1 : MCF7 cell line
Lane 2 : MDA-MB-231 cell line
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 87 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?MCF-7 lack LOXL2 expression (PMID: 19330836, PMID:12154058 and PMID: 27655685).
7.5% gel. Running: 80V, 15min; 140V, 40 min. Transfer: Semi-dry, 18 V, 60 min (Nitrocellulose membrane). Blocking: 5% non-fat milk in TBST, RT, 60 min. Primary antibody incubation: 4°C overnight. Washing: 5 ml TBST, 4 x 5 min. Exposure: Western blot HRP substrate.
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Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling LOXL2 with ab96233 at 1:500 (green). Cells were fixed in ice cold methanol for 5 minutes. Cells were co-stained with Hoechst 33342 (blue).
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IHC image of ab96233 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96233, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
All lanes : Anti-LOXL2 antibody (ab96233) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A431 cell lysate
Lane 3 : H1299 cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa
7.5% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (29)
ab96233 has been referenced in 29 publications.
- Choi SE et al. Inhibition of lysyl oxidase‑like 2 ameliorates folic acid‑induced renal tubulointerstitial fibrosis. Exp Ther Med 24:648 (2022). PubMed: 36168418
- Li Q et al. Deubiquitinase ZRANB1 drives hepatocellular carcinoma progression through SP1-LOXL2 axis. Am J Cancer Res 11:4807-4825 (2021). PubMed: 34765294
- Wang F et al. ZEB1 promotes colorectal cancer cell invasion and disease progression by enhanced LOXL2 transcription. Int J Clin Exp Pathol 14:9-23 (2021). PubMed: 33532019
- Zheng GL et al. Elevated LOXL2 expression by LINC01347/miR-328-5p axis contributes to 5-FU chemotherapy resistance of colorectal cancer. Am J Cancer Res 11:1572-1585 (2021). PubMed: 33948374
- Dey S et al. miR-29a Is Repressed by MYC in Pancreatic Cancer and Its Restoration Drives Tumor-Suppressive Effects via Downregulation of LOXL2. Mol Cancer Res 18:311-323 (2020). PubMed: 31662451