Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12733] to LOXL2 - C-terminal (HRP)
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameAnti-LOXL2 antibody [EPR12733] - C-terminal (HRP)
See all LOXL2 primary antibodies
DescriptionRabbit monoclonal [EPR12733] to LOXL2 - C-terminal (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human LOXL2 aa 750 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y4K0
- WB: U87-MG whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab202536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 87 kDa).|
Tissue specificityExpressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate.
Sequence similaritiesBelongs to the lysyl oxidase family.
Contains 4 SRCR domains.
modificationsThe lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- LOR 2 antibody
- LOR2 antibody
- LOX L2 antibody
Anti-LOXL2 antibody [EPR12733] - C-terminal (HRP) (ab202536) at 1/5000 dilution + U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Exposure time: 6 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202536 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab202536 has not yet been referenced specifically in any publications.