Overview

  • Product name

    Anti-LOXL2 antibody [EPR12733] - C-terminal (HRP)
    See all LOXL2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12733] to LOXL2 - C-terminal (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human LOXL2 aa 750 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y4K0

  • Positive control

    • WB: U87-MG whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab202536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 87 kDa).

Target

  • Tissue specificity

    Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate.
  • Sequence similarities

    Belongs to the lysyl oxidase family.
    Contains 4 SRCR domains.
  • Post-translational
    modifications

    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • LOR 2 antibody
    • LOR2 antibody
    • LOX L2 antibody
    • LOXL 2 antibody
    • LOXL2 antibody
    • LOXL2_HUMAN antibody
    • Lysyl oxidase homolog 2 antibody
    • Lysyl oxidase like 2 antibody
    • Lysyl oxidase like protein 2 antibody
    • Lysyl oxidase related 2 antibody
    • Lysyl oxidase related protein 2 antibody
    • Lysyl oxidase related protein WS9 14 antibody
    • Lysyl oxidase-like protein 2 antibody
    • Lysyl oxidase-related protein 2 antibody
    • Lysyl oxidase-related protein WS9-14 antibody
    • WS9 14 antibody
    see all

Images

  • Anti-LOXL2 antibody [EPR12733] - C-terminal (HRP) (ab202536) at 1/5000 dilution + U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 87 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 6 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab202536 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

     

References

ab202536 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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