Overview

  • Product name

  • Description

    Rabbit polyclonal to LOXL4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human LOXL4 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab99480)

  • Positive control

    • This antibody gave a positive signal in Human Testis and Skin Tissue Lysates. IF/ICC: A549 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab88186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 84 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    May modulate the formation of a collagenous extracellular matrix.
  • Tissue specificity

    Expressed in many tissues, the highest levels among the tissues studied being in the skeletal muscle, testis and pancreas. Expressed in cartilage.
  • Sequence similarities

    Belongs to the lysyl oxidase family.
    Contains 4 SRCR domains.
  • Post-translational
    modifications

    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4833426I20Rik antibody
    • FLJ21889 antibody
    • LOX L4 antibody
    • LOXC antibody
    • LOXL 4 antibody
    • LOXL4 antibody
    • LOXL4_HUMAN antibody
    • Lysyl oxidase homolog 4 antibody
    • Lysyl oxidase like 4 antibody
    • Lysyl oxidase like protein 4 antibody
    • Lysyl oxidase related C antibody
    • Lysyl oxidase related protein C antibody
    • Lysyl oxidase-like protein 4 antibody
    • Lysyl oxidase-related protein C antibody
    see all

Images

  • All lanes : Anti-LOXL4 antibody (ab88186) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human skin tissue lysate - total protein (ab30166)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 84 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 12 minutes


    The band observed at 80 kDa could potentially be a cleaved form of LOXL4. Abcam welcomes customer feedback.
  • IHC image of ab88186 staining in human testes formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88186, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ICC/IF image of ab88186 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88186, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Li R  et al. Exosome-mediated secretion of LOXL4 promotes hepatocellular carcinoma cell invasion and metastasis. Mol Cancer 18:18 (2019). Read more (PubMed: 30704479) »
  • Xie W  et al. Clinical significance of LOXL4 expression and features of LOXL4-associated protein-protein interaction network in esophageal squamous cell carcinoma. Amino Acids 51:813-828 (2019). Read more (PubMed: 30900087) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat Retinal Endothelial Cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Rat Retinal Endothelial Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Dec 22 2017

Answer

Thank you for your email. I have emailed the lab regarding the blocking experiment. I will let you know what I hear back. The immunogen peptide is available for sale as ab99480. Regarding the imunogen sequence, I would need to know which institution the customer is working at. Please let me know. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us. I have contacted the lab to check if experiments have been performed to check cross reactivity, but I can say from checking the homology for the immunogen with LOXL3 that there is only 50% homology with lots of gaps - therefore cross-reactivity seems rather unlikely. LOXL4 has a predicted MW of 84.5 kDa and LOXL3 has a MW of 83 kDa, which is very close and would be difficult to distinguish. Could you please let me know the following exp. details: 1) Which samples did the customer use? 2) Where were the band(s) seen? 3) What antibody dilution/incuation time was used? 4) What was used for the blocking and detection steps? I look forward to hear back and assist you further.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up