Anti-LPA-4 antibody (ab236731)
Key features and details
- Rabbit polyclonal to LPA-4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-LPA-4 antibody
See all LPA-4 primary antibodies -
Description
Rabbit polyclonal to LPA-4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human LPA-4 aa 1-100.
Database link: Q99677 -
Positive control
- WB: Mouse kidney tissue lysate. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 42 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Transduces a signal by increasing the intracellular calcium ions and by stimulating adenylyl cyclase activity. The rank order of potency for agonists of this receptor is 1-oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1-alkenyl-LPA. -
Tissue specificity
High expression in ovary. Not detected in the brain regions thalamus, putamen, caudate, frontal cortex, pons, hypothalamus and hippocampus. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2846 Human
- Entrez Gene: 78134 Mouse
- Omim: 300086 Human
- SwissProt: Q99677 Human
- SwissProt: Q8BLG2 Mouse
- Unigene: 522701 Human
- Unigene: 90147 Mouse
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Alternative names
- 5730485F04Rik antibody
- G protein coupled receptor 23 antibody
- G-protein coupled receptor 23 antibody
see all
Images
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Anti-LPA-4 antibody (ab236731) at 1/1000 dilution + Mouse kidney tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 42 kDa -
HepG2 (human liver hepatocellular carcinoma cell line) cells labeling LPA-4 (green) using ab236731 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236731 has not yet been referenced specifically in any publications.