Key features and details
- Rabbit polyclonal to LPCAT-2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-LPCAT-2 antibody
DescriptionRabbit polyclonal to LPCAT-2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
- WB: Human thyroid gland tissue lysate; NBT-II cell lysate. ICC/IF: A431 cells. IHC-P: Human thyroid gland tissue.
Previously labelled as Acyltransferase like 1.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 60 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPossesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-alkyl-phosphatidylcholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases.
PathwayLipid metabolism; phospholipid metabolism.
Sequence similaritiesBelongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
Contains 2 EF-hand domains.
DomainThe HXXXXD motif is essential for acyltransferase activity.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
- Information by UniProt
- 1 acylglycerophosphocholine O acyltransferase antibody
- 1 alkylglycerophosphocholine O acetyltransferase antibody
- 1-acylglycerophosphocholine O-acyltransferase antibody
Anti-LPCAT-2 antibody (ab224244) at 1/100 dilution + Human thyroid gland tissue lysate
Predicted band size: 60 kDa
A431 (human epidermoid carcinoma cell line) cells stained for LPCAT-2 (green) using ab224244 (4 µg/ml) in ICC/IF.
Paraffin embedded human thyroid gland tissue stained for LPCAT-2 with ab224244 (1/50 dilution) in immunohistochemical analysis.
All lanes : Anti-LPCAT-2 antibody (ab224244) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat cell line) cell lysate
Predicted band size: 60 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224244 has not yet been referenced specifically in any publications.