Recombinant
RabMAb

Recombinant Anti-LPCAT1 antibody [EPR19882] (ab214034)

Overview

  • Product name

    Anti-LPCAT1 antibody [EPR19882]
    See all LPCAT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19882] to LPCAT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human LPCAT1 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8NF37

  • Positive control

    • WB: Human fetal brain, testis, fetal spleen, fetal kidney and prostate cancer lysates; A431, A549 and PC-3 whole cell lysates. IHC-P: Human lung, liver cancer and lung cancer tissues. ICC/IF: A431 and A549 cells. Flow Cyt: A549 and A431 cells. IP: A549 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19882
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab214034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ICC/IF 1/250.
Flow Cyt 1/50.
IP 1/30.

Target

  • Function

    Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology.
  • Pathway

    Lipid metabolism; phospholipid metabolism.
  • Sequence similarities

    Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
    Contains 2 EF-hand domains.
  • Domain

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine.
    The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1 acylglycerophosphocholine O acyltransferase antibody
    • 1 alkylglycerophosphocholine O acetyltransferase antibody
    • 1-acylglycerophosphocholine O-acyltransferase antibody
    • 1-alkylglycerophosphocholine O-acetyltransferase antibody
    • Acetyl CoA:lyso PAF acetyltransferase antibody
    • Acetyl CoA:lyso platelet activating factor acetyltransferase antibody
    • Acetyl-CoA:lyso-PAF acetyltransferase antibody
    • Acetyl-CoA:lyso-platelet-activating factor acetyltransferase antibody
    • Acyl CoA:lysophosphatidylcholine acyltransferase 1 antibody
    • Acyltransferase like 2 antibody
    • Acyltransferase-like 2 antibody
    • AGPAT10 antibody
    • AGPAT9 antibody
    • AYTL1 antibody
    • AYTL2 antibody
    • FLJ12443 antibody
    • FLJ41609 antibody
    • LPC acyltransferase 1 antibody
    • LPC acyltransferase antibody
    • lpcat antibody
    • LPCAT-1 antibody
    • LPCAT1 antibody
    • Lyso PAF acetyltransferase antibody
    • Lyso-PAF acetyltransferase antibody
    • LysoPAFAT antibody
    • LysoPC acyltransferase 1 antibody
    • Lysophosphatidylcholine acyltransferase 1 antibody
    • PCAT1_HUMAN antibody
    • PFAAP3 antibody
    • Phosphonoformate immuno associated protein 3 antibody
    • Phosphonoformate immuno-associated protein 3 antibody
    see all

Images

  • Lanes 1-6 : Anti-LPCAT1 antibody [EPR19882] (ab214034) at 1/2000 dilution
    Lanes 7-8 : Anti-LPCAT1 antibody [EPR19882] (ab214034) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human testis lysate
    Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 5 : Human fetal spleen lysate
    Lane 6 : Human fetal kidney lysate
    Lane 7 : Human prostate cancer lysate
    Lane 8 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4/8: 4 seconds; Lane 5/6: 8 seconds; Lane 7: 3 minutes.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue labeling LPCAT1 with ab214034 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Cytoplasmic or granular staining on the type two alveolar epithelial cells of human lung is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling LPCAT1 with ab214034 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Cytoplasmic or granular staining on the liver cancer cells which contain abundant lipid drops is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling LPCAT1 with ab214034 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Cytoplasmic or granular staining on the lung cancer cells is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling LPCAT1 with ab214034 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on A431 cells. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling LPCAT1 with ab214034 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on A549 cells. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed A549 (Human lung carcinoma cell line) cells labeling LPCAT1 with ab214034 at 1/50 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed A431 (Human epidermoid carcinoma cell line) cells labeling LPCAT1 with ab214034 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • LPCAT1 was immunoprecipitated from 0.35 mg of A549 (Human lung carcinoma cell line) whole cell lysate with ab214034 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab214034 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: A549 whole cell lysate 10µg (Input).

    Lane 2: ab214034 IP in A549 whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab214034 in A549 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

ab214034 has not yet been referenced specifically in any publications.

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