Anti-LPCAT3 antibody (ab232958)
Key features and details
- Rabbit polyclonal to LPCAT3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LPCAT3 antibody
See all LPCAT3 primary antibodies -
Description
Rabbit polyclonal to LPCAT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Human LPCAT3 aa 100-250. Expressed in E.coli. N-terminal tag.
Database link: Q6P1A2 -
Positive control
- WB: Recombinant human LPCAT3 protein IHC-P: Human kidney, rectum, thyroid cancer and breast cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 56 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 56 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (LPCAT activity). Catalyzes also the conversion of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero-3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine or PS) (LPSAT activity). Has also weak lysophosphatidylethanolamine acyltransferase activity (LPEAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Seems to be the major enzyme contributing to LPCAT activity in the liver. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle. -
Tissue specificity
Highly expressed in liver, pancreas and adipose tissue. Very low expression in skeletal muscle and heart. Detected in neutrophils. -
Pathway
Lipid metabolism; phospholipid metabolism. -
Sequence similarities
Belongs to the membrane-bound acyltransferase family. -
Domain
The di-lysine motif confers endoplasmic reticulum localization. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 10162 Human
- SwissProt: Q6P1A2 Human
- Unigene: 655248 Human
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Alternative names
- 1-acylglycerophosphocholine O-acyltransferase antibody
- 1-acylglycerophosphoserine O-acyltransferase antibody
- LPCAT antibody
see all
Images
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Formalin-fixed, paraffin-embedded human kidney tissue stained for LPCAT3 using ab232958 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human rectum tissue stained for LPCAT3 using ab232958 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human thyroid cancer tissue stained for LPCAT3 using ab232958 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for LPCAT3 using ab232958 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Anti-LPCAT3 antibody (ab232958) at 2 µg/ml + Recombinant human LPCAT3 protein
Predicted band size: 56 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab232958 has been referenced in 2 publications.
- Zhang X et al. Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma. Cancer Commun (Lond) 42:287-313 (2022). PubMed: 35184419
- Zou Y et al. Plasticity of ether lipids promotes ferroptosis susceptibility and evasion. Nature 585:603-608 (2020). PubMed: 32939090