Key features and details
- Mouse polyclonal to LPGAT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LPGAT1 antibody
See all LPGAT1 primary antibodies
DescriptionMouse polyclonal to LPGAT1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
LPGAT1 (NP_055688.1, 1 a.a. ~ 370 a.a) full-length human protein.
- LPGAT1 transfected 293T lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab70076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 44 kDa).|
FunctionLysophoshatidylglycerol (LPG) specific acyltransferase that recognizes various acyl-CoAs and LPGs as substrates but demonstrates a clear preference for long chain saturated fatty acyl-CoAs and oleoyl-CoA as acyl donors. Prefers oleoyl-LPG over palmitoyl-LPG as an acyl receptor and oleoyl-CoA over lauroyl-CoA as an acyl donor.
Tissue specificityHighly expressed in liver and placenta. Also expressed in peripheral blood, lung, kidney and brain. Detected at lower levels in colon.
Sequence similaritiesBelongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
DomainThe HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- Acyl CoA:lysophosphatidylglycerol acyltransferase 1 antibody
- Acyl-CoA:lysophosphatidylglycerol acyltransferase 1 antibody
- FAM34A antibody
All lanes : Anti-LPGAT1 antibody (ab70076) at 1/500 dilution
Lane 1 : LPGAT1 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 44 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
ab70076 has not yet been referenced specifically in any publications.