Key features and details
- Rabbit polyclonal to LPLA2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LPLA2 antibody
See all LPLA2 primary antibodies
DescriptionRabbit polyclonal to LPLA2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
Recombinant fragment corresponding to Human LPLA2 aa 1-177.
Database link: AAH62605
- 293T, A431 and Jurkat cells and whole cell lysates; Human Colon carcinoma tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab111898 is purified by antigen-affinity chromatography.
Our Abpromise guarantee covers the use of ab111898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 47 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH8.0) may alternatively be used for heat-mediated antigen retrieval.|
|ICC/IF||1/100 - 1/500.|
FunctionHas transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid (By similarity). May have weak lysophospholipase activity.
Tissue specificityHighly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
Cellular localizationLysosome. Secreted.
- Information by UniProt
- 1 O acylceramide synthase antibody
- 1-O-acylceramide synthase antibody
- ACS antibody
Anti-LPLA2 antibody (ab111898) at 1/2000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE
ab111898 at 1/200 dilution staining LPLA2 in methanol-fixed A431 cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
ab111898 at 1/500 dilution staining LPLA2 in Formalin-fixed, Paraffin-embedded Human colon carcinoma by Immunohistochemistry.
ab111898 has not yet been referenced specifically in any publications.