Anti-LPLA2 antibody (ab111898)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-LPLA2 antibody
See all LPLA2 primary antibodies -
Description
Rabbit polyclonal to LPLA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
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Immunogen
Recombinant fragment corresponding to Human LPLA2 aa 1-177.
Database link: AAH62605 -
Positive control
- 293T, A431 and Jurkat cells and whole cell lysates; Human Colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab111898 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab111898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 47 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH8.0) may alternatively be used for heat-mediated antigen retrieval. | |
| ICC/IF | 1/100 - 1/500. |
Target
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Function
Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid (By similarity). May have weak lysophospholipase activity. -
Tissue specificity
Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Lysosome. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 282271 Cow
- Entrez Gene: 403403 Dog
- Entrez Gene: 23659 Human
- Entrez Gene: 192654 Mouse
- Entrez Gene: 361401 Rat
- Omim: 609362 Human
- SwissProt: Q8NCC3 Human
- SwissProt: Q8VEB4 Mouse
see all -
Alternative names
- 1 O acylceramide synthase antibody
- 1-O-acylceramide synthase antibody
- ACS antibody
see all
Images
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Western blot - Anti-LPLA2 antibody (ab111898)Anti-LPLA2 antibody (ab111898) at 1/2000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-LPLA2 antibody (ab111898)ab111898 at 1/200 dilution staining LPLA2 in methanol-fixed A431 cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPLA2 antibody (ab111898)ab111898 at 1/500 dilution staining LPLA2 in Formalin-fixed, Paraffin-embedded Human colon carcinoma by Immunohistochemistry.
Protocols
References
ab111898 has not yet been referenced specifically in any publications.
