Anti-LPLA2 antibody (ab111898)
Key features and details
- Rabbit polyclonal to LPLA2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LPLA2 antibody
See all LPLA2 primary antibodies -
Description
Rabbit polyclonal to LPLA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
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Immunogen
Recombinant fragment corresponding to Human LPLA2 aa 1-177.
Database link: AAH62605 -
Positive control
- 293T, A431 and Jurkat cells and whole cell lysates; Human Colon carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab111898 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab111898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 47 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH8.0) may alternatively be used for heat-mediated antigen retrieval. | |
| ICC/IF | 1/100 - 1/500. |
Target
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Function
Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid (By similarity). May have weak lysophospholipase activity. -
Tissue specificity
Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Lysosome. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 282271 Cow
- Entrez Gene: 403403 Dog
- Entrez Gene: 23659 Human
- Entrez Gene: 192654 Mouse
- Entrez Gene: 361401 Rat
- Omim: 609362 Human
- SwissProt: Q8NCC3 Human
- SwissProt: Q8VEB4 Mouse
see all -
Alternative names
- 1 O acylceramide synthase antibody
- 1-O-acylceramide synthase antibody
- ACS antibody
see all
Images
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Western blot - Anti-LPLA2 antibody (ab111898)Anti-LPLA2 antibody (ab111898) at 1/2000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-LPLA2 antibody (ab111898)ab111898 at 1/200 dilution staining LPLA2 in methanol-fixed A431 cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPLA2 antibody (ab111898)ab111898 at 1/500 dilution staining LPLA2 in Formalin-fixed, Paraffin-embedded Human colon carcinoma by Immunohistochemistry.
Protocols
References (0)
ab111898 has not yet been referenced specifically in any publications.
