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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Focal Adhesions
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RecombinantRabMAb

Recombinant Anti-LPP antibody [EPR6478] (ab126608)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Western blot - Anti-LPP antibody [EPR6478] (ab126608)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPP antibody [EPR6478] (ab126608)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPP antibody [EPR6478] (ab126608)
  • OI-RD Scanning - Anti-LPP antibody [EPR6478] (ab126608)
  • Anti-LPP antibody [EPR6478] (ab126608)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6478] to LPP
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-LPP antibody [EPR6478]
    See all LPP primary antibodies
  • Description

    Rabbit monoclonal [EPR6478] to LPP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human LPP aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Caco 2, HeLa, NIH 3T3 cell lysates; Human breast and kidney tissues.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.60 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6478
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions

Associated products

  • Alternative Versions

    • Anti-LPP antibody [EPR6478] - BSA and Azide free (ab248124)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • NIH/3T3 cytoplasmic extract lysate (ab14873)
    • NIH/3T3 nuclear extract lysate (ab14874)
    • HeLa membrane extract lysate (ab29547)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 66 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 66 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      May play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it may also be implicated in signaling events and activation of gene transcription. May be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. Also suggested to serve as a scaffold protein upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus.
    • Tissue specificity

      Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes.
    • Involvement in disease

      Note=A chromosomal aberration involving LPP is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with HMGA2 is shown in lipomas.
      Note=A chromosomal aberration involving LPP is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with HMGA2 is detected in pulmonary chondroid hamartomas.
      Note=A chromosomal aberration involving LPP is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with HMGA2 is also shown in one parosteal lipoma.
      Note=A chromosomal aberration involving LPP is associated with acute monoblastic leukemia. Translocation t(3;11)(q28;q23) with MLL is associated with acute monoblastic leukemia.
    • Sequence similarities

      Belongs to the zyxin/ajuba family.
      Contains 3 LIM zinc-binding domains.
    • Cellular localization

      Nucleus. Cytoplasm. Cell junction. Cell membrane. Found in the nucleus, in the cytoplasm and at cell adhesion sites. Shuttles between the cytoplasm and the nucleus. It has been found in sites of cell adhesion such as cell-to-cell contact and focal adhesion which are membrane attachment sites of cells to the extracellular matrix. Mainly nuclear when fused with HMGA2/HMGIC and MLL.
    • Target information above from: UniProt accession Q93052 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4026 Human
      • Entrez Gene: 210126 Mouse
      • Entrez Gene: 288010 Rat
      • Omim: 600700 Human
      • SwissProt: Q93052 Human
      • SwissProt: Q8BFW7 Mouse
      • SwissProt: Q5XI07 Rat
      • Unigene: 5724 Human
      • Alternative names

        • DKFZp779O0231 antibody
        • FLJ30652 antibody
        • FLJ41512 antibody
        • LIM domain containing preferred translocation partner in lipoma antibody
        • LIM domain-containing preferred translocation partner in lipoma antibody
        • LIM protein antibody
        • Lipoma preferred partner antibody
        • Lipoma-preferred partner antibody
        • lpp antibody
        • LPP_HUMAN antibody
        see all

      Images

      • Western blot - Anti-LPP antibody [EPR6478] (ab126608)
        Western blot - Anti-LPP antibody [EPR6478] (ab126608)
        All lanes : Anti-LPP antibody [EPR6478] (ab126608) at 1/10000 dilution

        Lane 1 : Caco 2 cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : NIH 3T3 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Predicted band size: 66 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPP antibody [EPR6478] (ab126608)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPP antibody [EPR6478] (ab126608)

        ab122608 at 1/250 staining LPP in paraffin embedded Human breast tissue by immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPP antibody [EPR6478] (ab126608)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPP antibody [EPR6478] (ab126608)

        ab122608 at 1/250 staining LPP in paraffin embedded Human kidney tissue by immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • OI-RD Scanning - Anti-LPP antibody [EPR6478] (ab126608)
        OI-RD Scanning - Anti-LPP antibody [EPR6478] (ab126608)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-LPP antibody [EPR6478] (ab126608)
        Anti-LPP antibody [EPR6478] (ab126608)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab126608? Please let us know so that we can cite the reference in this datasheet.

      ab126608 has been referenced in 1 publication.

      • Ngan E  et al. LPP is a Src substrate required for invadopodia formation and efficient breast cancer lung metastasis. Nat Commun 8:15059 (2017). IHC-P ; Mouse . PubMed: 28436416

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      I am looking into purchasing the following antibody :  Anti-LPP antibody [EPR6478] (ab126608), but would like to get some additional information. I am investigating different isoforms of LPP which differ in the N-terminal of the protein. I was wondering if you can tell me whether this antibody was raised against an epitope within the first 50 amino acids of full length LPP?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 15 2013

      Answer

      This product has been raised from a peptide corresponding to the N terminal region of human LPP.

      Read More

      Abcam Scientific Support

      Answered on Mar 15 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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