Overview

  • Product name

    Anti-LPP antibody [EPR6479(2)]
    See all LPP primary antibodies
  • Description

    Rabbit monoclonal [EPR6479(2)] to LPP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region in Human LPP (UniProt: Q93052).

  • Positive control

    • Caco2, 293T and HeLa cell lysates; HeLa cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6479(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 66 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      May play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it may also be implicated in signaling events and activation of gene transcription. May be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. Also suggested to serve as a scaffold protein upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus.
    • Tissue specificity

      Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes.
    • Involvement in disease

      Note=A chromosomal aberration involving LPP is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with HMGA2 is shown in lipomas.
      Note=A chromosomal aberration involving LPP is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with HMGA2 is detected in pulmonary chondroid hamartomas.
      Note=A chromosomal aberration involving LPP is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with HMGA2 is also shown in one parosteal lipoma.
      Note=A chromosomal aberration involving LPP is associated with acute monoblastic leukemia. Translocation t(3;11)(q28;q23) with MLL is associated with acute monoblastic leukemia.
    • Sequence similarities

      Belongs to the zyxin/ajuba family.
      Contains 3 LIM zinc-binding domains.
    • Cellular localization

      Nucleus. Cytoplasm. Cell junction. Cell membrane. Found in the nucleus, in the cytoplasm and at cell adhesion sites. Shuttles between the cytoplasm and the nucleus. It has been found in sites of cell adhesion such as cell-to-cell contact and focal adhesion which are membrane attachment sites of cells to the extracellular matrix. Mainly nuclear when fused with HMGA2/HMGIC and MLL.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp779O0231 antibody
      • FLJ30652 antibody
      • FLJ41512 antibody
      • LIM domain containing preferred translocation partner in lipoma antibody
      • LIM domain-containing preferred translocation partner in lipoma antibody
      • LIM protein antibody
      • Lipoma preferred partner antibody
      • Lipoma-preferred partner antibody
      • lpp antibody
      • LPP_HUMAN antibody
      see all

    Images

    • All lanes : Anti-LPP antibody [EPR6479(2)] (ab133651) at 1/1000 dilution

      Lane 1 : Caco2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 66 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?

    • Flow cytometrical analysis of LPP in permeabilized HeLa cells labelled with ab133651 at a 1/10 dilution (red), or with a Rabbit IgG control (negative) (green).

    References

    ab133651 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133651.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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