Key features and details
- Rabbit polyclonal to LPPR2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LPPR2 antibody
DescriptionRabbit polyclonal to LPPR2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- IHC-P: Human liver tissue. WB: Human liver tissue lysate. ICC/IF: U-251 MG cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab253014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the PA-phosphatase related phosphoesterase family.
- Information by UniProt
- Lipid phosphate phosphatase-related protein type 2 antibody
- LPPR2 antibody
- LPPR2_HUMAN antibody
PFA-fixed, Triton X-100 permeabilized U-251MG (Human brain glioma cell line) cells cells stained for LPPR2 (green) using ab253014 at 4 µg/ml in ICC/IF.
Paraffin-embedded human liver tissue stained for LPPR2 using ab253014 at 1/1000 dilution in immunohistochemical analysis.
All lanes : Anti-LPPR2 antibody (ab253014) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab253014 has not yet been referenced specifically in any publications.