Overview

  • Product name

    LPS Extraction Kit
  • Species reactivity

    Reacts with: Bacteria
  • Product overview

    LPS Extraction Kit (ab239718) uses bacterial membrane lysis buffer and protein digestion to yield micrograms of LPS from bacterial culture (approximately 1-4% of dry weight). This kit does not use chloroform or phenol like traditional methods, and it will yield pure LPS in less than 2 hours that can be easily characterized and quantified.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 tests
    LPS Isolation Buffer 1 x 100ml
    Proteinase K (20 mg/ml) 1 x 0.6ml
  • Relevance

    The outer membrane of gram-negative bacteria contains lipopolysaccharide (LPS), a low molecular weight carbohydrate with a molecular mass of 10-20 kDa. It is heterogeneous, and composed of O antigen (a repeating glycan polymer), core oligosaccharide (which links the O antigen to Lipid A - the third component, and non-carbohydrate components such as phosphate and amino acids groups. Lipid A, has multiple fatty acids which serve to anchor LPS into the bacterial membrane allowing the O antigen and core oligosaccharide to protrude and contributes to a major part of the toxicity of gram-negative bacteria. Also known as endotoxin, when consumed by animals, LPS induces a strong inflammatory response and/or sepsis.

Images

  • LPS is a 10-20 kDa carbohydrate that is associated with low molecular weight proteins and gives a characteristic ladder banding pattern in coomassie blue stained SDS-PAGE gels. Lane 1 represents E.coli lysate prior to Proteinase K digestion.  Lane 2 illustrates lysate after Proteinase K digestion of proteins. MM: molecular weight marker.

Protocols

References

ab239718 has not yet been referenced specifically in any publications.

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