Key features and details
- Rabbit polyclonal to LRAT
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LRAT antibody
See all LRAT primary antibodies
DescriptionRabbit polyclonal to LRAT
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to amino acids within an internal region of Human LRAT.
- COLO205 cell lysates
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Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab137304 was affinity purified from Rabbit antiserum by affinity chromatography using epitope specific peptide.
Our Abpromise guarantee covers the use of ab137304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 26 kDa.|
FunctionTransfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments.
Tissue specificityHepatic stellate cells and endothelial cells (at protein level). Found at high levels in testis and liver, followed by retinal pigment epithelium, small intestine, prostate, pancreas and colon. Low expression observed in brain. In fetal tissues, expressed in retinal pigment epithelium and liver, and barely in the brain.
PathwayCofactor metabolism; retinol metabolism.
Involvement in diseaseDefects in LRAT are a cause of Leber congenital amaurosis type 14 (LCA14) [MIM:613341]. It is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus.
Sequence similaritiesBelongs to the H-rev107 family.
Cellular localizationEndoplasmic reticulum membrane. Rough endoplasmic reticulum. Endosome > multivesicular body. Cytoplasm > perinuclear region. Present in the rough endoplasmic reticulum and multivesicular body in hepatic stellate cells. Present in the rough endoplasmic reticulum and perinuclear region in endothelial cells.
- Information by UniProt
- LCA14 antibody
- Lecithin retinol acyltransferase (phosphatidylcholine retinol O acyltransferase) antibody
- Lecithin retinol acyltransferase antibody
ab137304 has been referenced in 2 publications.
- Samuel W et al. Appropriately differentiated ARPE-19 cells regain phenotype and gene expression profiles similar to those of native RPE cells. Mol Vis 23:60-89 (2017). WB ; Human . PubMed: 28356702
- Muñiz A et al. Retinoid uptake, processing, and secretion in human iPS-RPE support the visual cycle. Invest Ophthalmol Vis Sci 55:198-209 (2014). PubMed: 24255038