Overview

  • Product name

  • Description

    Rabbit polyclonal to LRAT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from internal sequence of Human LRAT.

  • Positive control

    • Human tonsil tissue; COLO205 cell extract; HUVEC cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab166784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 26 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments.
  • Tissue specificity

    Hepatic stellate cells and endothelial cells (at protein level). Found at high levels in testis and liver, followed by retinal pigment epithelium, small intestine, prostate, pancreas and colon. Low expression observed in brain. In fetal tissues, expressed in retinal pigment epithelium and liver, and barely in the brain.
  • Pathway

    Cofactor metabolism; retinol metabolism.
  • Involvement in disease

    Defects in LRAT are a cause of Leber congenital amaurosis type 14 (LCA14) [MIM:613341]. It is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus.
  • Sequence similarities

    Belongs to the H-rev107 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Rough endoplasmic reticulum. Endosome > multivesicular body. Cytoplasm > perinuclear region. Present in the rough endoplasmic reticulum and multivesicular body in hepatic stellate cells. Present in the rough endoplasmic reticulum and perinuclear region in endothelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • LCA14 antibody
    • Lecithin retinol acyltransferase (phosphatidylcholine retinol O acyltransferase) antibody
    • Lecithin retinol acyltransferase antibody
    • LRAT antibody
    • LRAT_HUMAN antibody
    • Phosphatidylcholine retinol O acyltransferase antibody
    • Phosphatidylcholine--retinol O-acyltransferase antibody
    see all

Images

  • All lanes : Anti-LRAT antibody (ab166784) at 1/500 dilution

    Lane 1 : COLO205 cell extract
    Lane 2 : COLO205 cell extract with immunizing peptide

    Predicted band size: 26 kDa

  • Immunofluorescent analysis of HUVEC cells labeling LRAT with ab166784 at 1/100 dilution in the presence (right panel) or absence (left panel) of immunizing peptide.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling LRAT with ab166784 at 5 µg/ml.

References

ab166784 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lung)
Permeabilization
No
Specification
Lung
Blocking step
1%BSA/1.5% Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 08 2017

Answer

Vielen Dank für Ihre Anfrage.

Ich freue mich Ihnen bestätigen zu können, dass unsere Labor die folgende Antigendemaskierungsprozedur verwendet hat:

Antigen retrieval:

Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.



Als Lysispuffer im WB wurde standard RIPA Puffer eingesetzt, der 0.1% SDS enthaelt:

50 mM Tris HCl pH 8
150 mM NaCl
1% NP-40
0.5% sodium deoxycholate
0.1% SDS



The 10% sodium deoxycholate stock solution (5 g into 50 ml) must be protected from light.

The 100 mM EDTA stock solution is made with 1.86 g into 40 ml H2O and then add NaOH to dissolve and adjust pH to 7.4. Finally, adjust the total volume to 50 ml). Store the buffer at 4°C.

Ich hoffe, diese Information ist hilfreich und wünsche Ihnen viel Erfolg bei Ihren Versuchen.

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