Anti-LRAT antibody (ab166784)
Key features and details
- Rabbit polyclonal to LRAT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LRAT antibody
See all LRAT primary antibodies -
Description
Rabbit polyclonal to LRAT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from internal sequence of Human LRAT.
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Positive control
- Human tonsil tissue; COLO205 cell extract; HUVEC cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 26 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 26 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments. -
Tissue specificity
Hepatic stellate cells and endothelial cells (at protein level). Found at high levels in testis and liver, followed by retinal pigment epithelium, small intestine, prostate, pancreas and colon. Low expression observed in brain. In fetal tissues, expressed in retinal pigment epithelium and liver, and barely in the brain. -
Pathway
Cofactor metabolism; retinol metabolism. -
Involvement in disease
Defects in LRAT are a cause of Leber congenital amaurosis type 14 (LCA14) [MIM:613341]. It is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. -
Sequence similarities
Belongs to the H-rev107 family. -
Cellular localization
Endoplasmic reticulum membrane. Rough endoplasmic reticulum. Endosome > multivesicular body. Cytoplasm > perinuclear region. Present in the rough endoplasmic reticulum and multivesicular body in hepatic stellate cells. Present in the rough endoplasmic reticulum and perinuclear region in endothelial cells. - Information by UniProt
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Database links
- Entrez Gene: 9227 Human
- Omim: 604863 Human
- SwissProt: O95237 Human
- Unigene: 658427 Human
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Alternative names
- LCA14 antibody
- Lecithin retinol acyltransferase (phosphatidylcholine retinol O acyltransferase) antibody
- Lecithin retinol acyltransferase antibody
see all
Images
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All lanes : Anti-LRAT antibody (ab166784) at 1/500 dilution
Lane 1 : COLO205 cell extract
Lane 2 : COLO205 cell extract with immunizing peptide
Predicted band size: 26 kDa -
Immunofluorescent analysis of HUVEC cells labeling LRAT with ab166784 at 1/100 dilution in the presence (right panel) or absence (left panel) of immunizing peptide.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling LRAT with ab166784 at 5 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab166784 has been referenced in 1 publication.
- McCormick R et al. Optimisation of a Novel Bio-Substrate as a Treatment for Atrophic Age-Related Macular Degeneration. Front Bioeng Biotechnol 8:456 (2020). PubMed: 32500067