Key features and details
- Rabbit polyclonal to LRBA/BGL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LRBA/BGL antibody
See all LRBA/BGL primary antibodies
DescriptionRabbit polyclonal to LRBA/BGL
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human LRBA/BGL aa 1125-1175. The exact sequence is proprietary. (NP_006717.2).
Database link: P50851
- WB: HeLa, HEK-293T and Jurkat whole cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab191174 was affinity purified using an epitope specific to LRBA/BGL immobilized on solid support.
Our Abpromise guarantee covers the use of ab191174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 319 kDa.|
FunctionMay be involved in coupling signal transduction and vesicle trafficking to enable polarized secretion and/or membrane deposition of immune effector molecules.
Sequence similaritiesContains 1 BEACH domain.
Contains 6 WD repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCell membrane. Endoplasmic reticulum. Golgi apparatus > trans-Golgi network. Lysosome.
- Information by UniProt
- Beige-like protein antibody
- BGL antibody
- C80285 antibody
All lanes : Anti-LRBA/BGL antibody (ab191174) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Developed using the ECL technique.
Predicted band size: 319 kDa
Exposure time: 30 seconds
Lysates prepared using RIPA lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab191174 has not yet been referenced specifically in any publications.