Key features and details
- Rabbit polyclonal to LRCH1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LRCH1 antibody
DescriptionRabbit polyclonal to LRCH1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: HeLa and MCF7 cell lysate. IHC-P: Human kidney tissue. ICC/IF: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab234875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 81 kDa.|
Sequence similaritiesContains 1 CH (calponin-homology) domain.
Contains 9 LRR (leucine-rich) repeats.
- Information by UniProt
- Calponin homology domain-containing protein 1 antibody
- CHDC1 antibody
- KIAA1016 antibody
All lanes : Anti-LRCH1 antibody (ab234875) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 81 kDa
Paraffin-embedded human kidney tissue stained for LRCH1 using ab234875 at 1/100 dilution in immunohistochemical analysis.
MCF7 (Human breast adenocarcinoma cell line) cells labeling LRCH1 using ab234875 at 1/100 dilution in ICC/IF. Secondary antibody used was an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234875 has not yet been referenced specifically in any publications.