Product nameAnti-LRCH2 antibody
DescriptionRabbit polyclonal to LRCH2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to amino acids 348-489 of Human LRCH2 (Q5VUJ6).
- RT4 and U-251 MG cell lysates; Human stomach tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/350. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
Sequence similaritiesContains 1 CH (calponin-homology) domain.
Contains 9 LRR (leucine-rich) repeats.
- Information by UniProt
- KIAA1495 antibody
- Leucine-rich repeat and calponin homology domain-containing protein 2 antibody
- LRCH2 antibody
- LRCH2_HUMAN antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleoli. Recommended concentration of ab121182 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-LRCH2 antibody (ab121182) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
ab121182 at 1/350 dilution staining LRCH2 in Paraffin-emebedded Human stomach tissue by Immunohistochemistry.
ab121182 has not yet been referenced specifically in any publications.