Key features and details
- Rabbit polyclonal to LRFN1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-LRFN1 antibody
DescriptionRabbit polyclonal to LRFN1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
18 amino acid peptide near the C terminus of Human LRFN1 (Q9P244).
- Human brain lysate, Mouse brain tissue
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 82 kDa.|
|IHC-P||Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
FunctionPromotes neurite outgrowth in hippocampal neurons. Involved in the regulation and maintenance of excitatory synapses. Induces the clustering of excitatory postsynaptic proteins, including DLG4, DLGAP1, GRIA1 and GRIN1.
Sequence similaritiesBelongs to the LRFN family.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 7 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
DomainThe PDZ-binding motif is required for neurite outgrowth promotion and for DLG1-, DLG3- and DLG4-binding.
Cellular localizationMembrane. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Detected in excitatory, but not inhibitory, synaptic plasma membrane.
- Information by UniProt
- KIAA1484 antibody
- Leucine rich repeat and fibronectin type III domain containing 1 antibody
- Leucine rich repeat and fibronectin type III domain containing protein 1 antibody
Immunofluorescence of LRFN1 in mouse brain tissue with ab106365 at 20 ug/mL.
Lane 1 : Anti-LRFN1 antibody (ab106365) at 1 µg/ml
Lane 2 : Anti-LRFN1 antibody (ab106365) at 2 µg/ml
All lanes : Human brain lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 82 kDa
ab106365 at 2.5 µg/ml staining LRFN1 in Mouse brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
ab106365 has not yet been referenced specifically in any publications.