Overview

  • Product name

    Anti-LRFN5 antibody
  • Description

    Rabbit polyclonal to LRFN5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 16 aa from near the C-terminus of Human LRFN5 (Q96NI6).

  • Positive control

    • EL4 cell lysate, Mouse brain tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months).
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 79 kDa.

Target

  • Function

    Cell adhesion molecule that mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Promotes neurite outgrowth in hippocampal neurons.
  • Sequence similarities

    Belongs to the LRFN family.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Domain

    Lacks a cytoplasmic PDZ-binding motif, which has been implicated in function of related LRFN proteins.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C14orf146 antibody
    • DKFZp686G0210 antibody
    • Fibronectin type III immunoglobulin and leucine rich repeat domains 8 antibody
    • FIGLER8 antibody
    • FLJ30803 antibody
    • leucine rich repeat and fibronectin type III domain containing 5 antibody
    • Leucine-rich repeat and fibronectin type-III domain-containing protein 5 antibody
    • Lrfn5 antibody
    • LRFN5_HUMAN antibody
    • SALM5 antibody
    see all

Images

  • Lane 1 : Anti-LRFN5 antibody (ab106370) at 0.5 µg/ml
    Lane 2 : Anti-LRFN5 antibody (ab106370) at 1 µg/ml

    All lanes : EL4 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 79 kDa

  • ab106370, at 5 ug/ml, staining LRFN5 in Mouse brain tissue by immunohistochemistry.

References

This product has been referenced in:

  • Burch A  et al. A novel synaptic junction preparation for the identification and characterization of cleft proteins. PLoS One 12:e0174895 (2017). Read more (PubMed: 28362857) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply.

The discount code is valid for untested species / applications. Western Blot is a tested application for the three of them, so I am afraid the discount code would not be applicable in these cases. You can still send us your results and earn Abpoints for sharing them with us. To read more about Abpoints:

https://www.abcam.com/index.html?pageconfig=loyalty_about&viapagetrap=abpoints

So, if you send us further images / reviews, you can earn extra Abpoints exchangeable for rewards / discounts.

If you wish to test any of the antibodies in a new application, please let me know before purchasing it so I can raise the discount code, and once you have submitted your results to our website, this code is activated and you can then place your free order.

The next link contains useful information about the Abreview system:

https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews

I hope this information was helpful. If not please let me know so I can help you further.

Read More

Answer

Thank you for contacting us.

Ab106370 has been used in WB and IHC with paraffin embedded sections in Mouse, Rat and Human species. Ab105169 has successfully worked in WB and IHC in both frozen and paraffin embedded sections and ab87165 has only been characterized by WB in Human (although due to high sequence homology it is also expected to react with Mouse, Rat, Chicken, Cow and Dog).

The fact that there aren’t any other applications stated on the datasheet does not mean the antibody won’t work in them, it just means we have not tested it, and therefore we don’t have data to support it.

If you are interested in participating in our antibodies evaluation system (Abreview), and test the antibodies in new applications (not stated on the datasheet) I will be happy to offer a discount code for a future purchase if you submit the results (in a form of an Abreview) to our website.

It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please let me know before placing the order.

If you want to read more about the Abreview system, please have a look at the next link:

https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews

Please let me know if you would be interested in this program, and if you have any other doubts about it. I am happy to help, and clarify it further if necessary.

Read More

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