Overview

  • Product name

  • Description

    Rabbit polyclonal to LRG1/LRG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human LRG1/LRG aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab129215)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Liver; Human Plasma Total protein; Human Spleen; Human Small Intestine; Human Lung.
  • General notes

     This product was previously labelled as LRG1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).

Target

  • Tissue specificity

    Plasma.
  • Sequence similarities

    Contains 8 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1300008B03Rik antibody
    • 2310031E04Rik antibody
    • A2GL antibody
    • A2GL_HUMAN antibody
    • HMFT1766 antibody
    • Leucine rich alpha 2 glycoprotein antibody
    • Leucine-rich alpha-2-glycoprotein 1 antibody
    • Leucine-rich alpha-2-glycoprotein antibody
    • Leucine-rich alpha-2-glycoprotein precursor antibody
    • LRG antibody
    • LRG1 antibody
    see all

Images

  • All lanes : Anti-LRG1/LRG antibody (ab128880) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human Plasma Total Protein Lysate
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 5 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    LRG1/LRG contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

ab128880 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your reply.

I have had a look at all the anti-LRG1 antibodies we have to see if any of them might be able to pick up this difference in glycosylation.

The only one I would suggest might be worth trying is the ab63836. This immunogen used to raise this antibody does cover the region with the glycosylation site 37, however, there is no knowing whether the antibody detects this precise region or not.

Alternatively, the ab105312 covers the region 35-84 of LRG1 and may therefore be able to pick up the glycosylation at 37 or 79, however as it is a rabbit polyclonal (as is ab63836), it may be that there are a mixture of antibodies, detecting different epitopes.

Could you detect the glycosylation by treating the sample with enzymes to remove the glycosylation and performing a western blotting comparing the sample with unteated sample (there shouldbe a shift in size of the bands)?

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

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