Key features and details
- Rabbit polyclonal to LRG1/LRG
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-LRG1/LRG antibody
See all LRG1/LRG primary antibodies
DescriptionRabbit polyclonal to LRG1/LRG
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Recombinant fragment (His-tag) corresponding to Mouse LRG1/LRG aa 136-342. N-terminal His-tag and GST-tag. Expressed in E.coli.
STLVLRENQLREVSAQWLQGLDALGHLDLAENQLSSLPSGLLASLGALHT LDLGYNLLESLPEGLLRGPRRLQRLHLEGNRLQRLEDSLLAPQPFLRVLF LNDNQLVGVATGSFQGLQHLDMLDLSNNSLSSTPPGLWAFLGRPTRDMQD GFDISHNPWICDKNLADLCRWLVANRNKMFSQNDTRCAGPEAMKGQRLLD VAELGSL
Database link: Q91XL1
- WB: Recombinant mouse LRG1/LRG; MouseSP2/0 cell lysate; Mouse liver and pancreas lysates. IHC-P: Mouse stomach, esophagus and uterus tissue.
This product was previously labelled as LRG1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 38 kDa.|
Sequence similaritiesContains 8 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
- Information by UniProt
- 1300008B03Rik antibody
- 2310031E04Rik antibody
- A2GL antibody
Anti-LRG1/LRG antibody (ab231188) at 3 µg/ml + Recombinant mouse LRG1/LRG
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 38 kDa
All lanes : Anti-LRG1/LRG antibody (ab231188) at 3 µg/ml
Lane 1 : Mouse SP2/0 cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse pancreas lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 38 kDa
Formalin-fixed, paraffin-embedded mouse stomach tissue stained for LRG1/LRG using ab231188 at 20 mg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse esophagus tissue stained for LRG1/LRG using ab231188 at 20 mg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse uterus tissue stained for LRG1/LRG using ab231188 at 20 mg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231188 has been referenced in 1 publication.
- Gao Y et al. Mechanical strain promotes skin fibrosis through LRG-1 induction mediated by ELK1 and ERK signalling. Commun Biol 2:359 (2019). PubMed: 31602408