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Immunology Secreted Molecules Other secreted molecules
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RecombinantRabMAb

Recombinant Anti-LRG1/LRG antibody [EPR12362] (ab178698)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (8)

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Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
  • Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
  • Anti-LRG1/LRG antibody [EPR12362] (ab178698)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12362] to LRG1/LRG
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Recombinant Human LRG1/LRG protein (ab182835)
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Overview

  • Product name

    Anti-LRG1/LRG antibody [EPR12362]
    See all LRG1/LRG primary antibodies
  • Description

    Rabbit monoclonal [EPR12362] to LRG1/LRG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    within Human LRG1/LRG aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P02750

  • Positive control

    • WB: Human plasma and Human serum lysates;. IHC-P: human liver carcinoma and lung tissue.
  • General notes

     

     

     This product was previously labelled as LRG1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12362
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Secreted Molecules
    • Other secreted molecules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Other ECM Proteins

Associated products

  • Alternative Versions

    • Anti-LRG1/LRG antibody [EPR12362] - BSA and Azide free (ab240209)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human LRG1/LRG protein (ab182835)
  • Related Products

    • Recombinant Human LRG1/LRG protein (denatured) (ab171596)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for ICC.
  • Target

    • Tissue specificity

      Plasma.
    • Sequence similarities

      Contains 8 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02750 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 116844 Human
      • Omim: 611289 Human
      • SwissProt: P02750 Human
      • Unigene: 655559 Human
      • Alternative names

        • 1300008B03Rik antibody
        • 2310031E04Rik antibody
        • A2GL antibody
        • A2GL_HUMAN antibody
        • HMFT1766 antibody
        • Leucine rich alpha 2 glycoprotein antibody
        • Leucine-rich alpha-2-glycoprotein 1 antibody
        • Leucine-rich alpha-2-glycoprotein antibody
        • Leucine-rich alpha-2-glycoprotein precursor antibody
        • LRG antibody
        • LRG1 antibody
        see all

      Images

      • Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
        Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
        Anti-LRG1/LRG antibody [EPR12362] (ab178698) at 1/10000 dilution (Purified) + Human plasma lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 38 kDa
        Observed band size: 50 kDa why is the actual band size different from the predicted?



        The band observed is consistent with what has been described in PMID: 28081565

      • Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
        Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
        All lanes : Anti-LRG1/LRG antibody [EPR12362] (ab178698) at 1/1000 dilution ((unpurified))

        Lane 1 : Human plasma lysate
        Lane 2 : Human serum lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 38 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling LRG1/LRG with purified ab178698 at 1/200 dilution (0.57 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)

        Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LRG1/LRG with unpurified ab178698 at 1/100 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-LRG1/LRG antibody [EPR12362] (ab178698)
        Anti-LRG1/LRG antibody [EPR12362] (ab178698)

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (8)

      Publishing research using ab178698? Please let us know so that we can cite the reference in this datasheet.

      ab178698 has been referenced in 8 publications.

      • Hong Q  et al. LRG1 May Accelerate the Progression of ccRCC via the TGF-ß Pathway. Biomed Res Int 2020:1285068 (2020). PubMed: 32337221
      • Jin Z  et al. The Prognostic Impact of Leucine-Rich a-2-Glycoprotein-1 in Cholangiocarcinoma and Its Association With the IL-6/TGF-ß1 Axis. J Surg Res 252:147-155 (2020). PubMed: 32278969
      • Otsuru T  et al. Epithelial-mesenchymal transition via transforming growth factor beta in pancreatic cancer is potentiated by the inflammatory glycoprotein leucine-rich alpha-2 glycoprotein. Cancer Sci 110:985-996 (2019). PubMed: 30575211
      • Ban Z  et al. LRG-1 enhances the migration of thyroid carcinoma cells through promotion of the epithelial-mesenchymal transition by activating MAPK/p38 signaling. Oncol Rep 41:3270-3280 (2019). PubMed: 31002347
      • Li Z  et al. Exosomal Leucine-Rich-Alpha2-Glycoprotein 1 Derived from Non-Small-Cell Lung Cancer Cells Promotes Angiogenesis via TGF-ß Signal Pathway. Mol Ther Oncolytics 14:313-322 (2019). PubMed: 31528707
      • Gao Y  et al. Mechanical strain promotes skin fibrosis through LRG-1 induction mediated by ELK1 and ERK signalling. Commun Biol 2:359 (2019). PubMed: 31602408
      • Xiao S & Zhu H Leucine-Rich Alpha-2-Glycoprotein1 Gene Interferes with Regulation of Apoptosis in Leukemia KASUMI-1 Cells. Med Sci Monit 24:8348-8356 (2018). PubMed: 30455412
      • Druhan LJ  et al. Leucine Rich a-2 Glycoprotein: A Novel Neutrophil Granule Protein and Modulator of Myelopoiesis. PLoS One 12:e0170261 (2017). Functional Studies ; Human . PubMed: 28081565

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LRG1 antibody [EPR12362]

      Excellent
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (lung)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate
      Permeabilization
      No
      Specification
      lung
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
      Fixative
      Formalin
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 10 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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