Overview

  • Product name

    Anti-LRG1/LRG antibody [EPR12362]
    See all LRG1/LRG primary antibodies
  • Description

    Rabbit monoclonal [EPR12362] to LRG1/LRG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    within Human LRG1/LRG aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P02750

  • Positive control

    • Human plasma and Human serum lysates; Human lung tissue.
  • General notes

     

     

     This product was previously labelled as LRG1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Tissue specificity

      Plasma.
    • Sequence similarities

      Contains 8 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1300008B03Rik antibody
      • 2310031E04Rik antibody
      • A2GL antibody
      • A2GL_HUMAN antibody
      • HMFT1766 antibody
      • Leucine rich alpha 2 glycoprotein antibody
      • Leucine-rich alpha-2-glycoprotein 1 antibody
      • Leucine-rich alpha-2-glycoprotein antibody
      • Leucine-rich alpha-2-glycoprotein precursor antibody
      • LRG antibody
      • LRG1 antibody
      see all

    Images

    • All lanes : Anti-LRG1/LRG antibody [EPR12362] (ab178698) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human serum lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 38 kDa

    • Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LRG1/LRG with ab178698 at 1/100 dilution.

    • Western blot analysis on immunoprecipitation pellet from (1) Human plasma lysate or (2) 1X PBS (negative control) immunoprecipitated using ab178698 at 1/10 dilution, with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:

    • Otsuru T  et al. Epithelial-mesenchymal transition via transforming growth factor beta in pancreatic cancer is potentiated by the inflammatory glycoprotein leucine-rich alpha-2 glycoprotein. Cancer Sci 110:985-996 (2019). Read more (PubMed: 30575211) »
    • Xiao S & Zhu H Leucine-Rich Alpha-2-Glycoprotein1 Gene Interferes with Regulation of Apoptosis in Leukemia KASUMI-1 Cells. Med Sci Monit 24:8348-8356 (2018). Read more (PubMed: 30455412) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate
    Permeabilization
    No
    Specification
    lung
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    Formalin

    Abcam user community

    Verified customer

    Submitted Mar 10 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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