Recombinant Anti-LRG1/LRG antibody [EPR12362] (ab178698)
![Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)](https://www.abcam.com/ps/products/178/ab178698/Images/ab178698-365429-anti-lrg1-lrg-antibody-epr12362-westernblot-plasma-human.jpg "Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12362] to LRG1/LRG
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-LRG1/LRG antibody [EPR12362]
See all LRG1/LRG primary antibodies -
Description
Rabbit monoclonal [EPR12362] to LRG1/LRG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
within Human LRG1/LRG aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P02750 -
Positive control
- WB: Human plasma and Human serum lysates;. IHC-P: human liver carcinoma and lung tissue.
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General notes
This product was previously labelled as LRG1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12362 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Tissue specificity
Plasma. -
Sequence similarities
Contains 8 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 116844 Human
- Omim: 611289 Human
- SwissProt: P02750 Human
- Unigene: 655559 Human
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Alternative names
- 1300008B03Rik antibody
- 2310031E04Rik antibody
- A2GL antibody
see all
Images
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Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)Anti-LRG1/LRG antibody [EPR12362] (ab178698) at 1/10000 dilution (Purified) + Human plasma lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?The band observed is consistent with what has been described in PMID: 28081565
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![Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)](https://www.abcam.com/ps/products/178/ab178698/Images/ab178698-206907-anti-lrg1-antibody-epr12362-western-blot.jpg)
Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)All lanes : Anti-LRG1/LRG antibody [EPR12362] (ab178698) at 1/1000 dilution ((unpurified))
Lane 1 : Human plasma lysate
Lane 2 : Human serum lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)](https://www.abcam.com/ps/products/178/ab178698/Images/ab178698-4-anti-lrg1-lrg-antibody-epr12362-immunohistochemistry-liver-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling LRG1/LRG with purified ab178698 at 1/200 dilution (0.57 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)](https://www.abcam.com/ps/products/178/ab178698/Images/ab178698-206908-anti-lrg1-antibody-epr12362-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LRG1/LRG with unpurified ab178698 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-LRG1/LRG antibody [EPR12362] (ab178698)](https://www.abcam.com/ps/products/178/ab178698/Images/ab178698-6-benefits-of-recombinant-antibodies.png)
Anti-LRG1/LRG antibody [EPR12362] (ab178698)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab178698 has been referenced in 8 publications.
- Hong Q et al. LRG1 May Accelerate the Progression of ccRCC via the TGF-ß Pathway. Biomed Res Int 2020:1285068 (2020). PubMed: 32337221
- Jin Z et al. The Prognostic Impact of Leucine-Rich a-2-Glycoprotein-1 in Cholangiocarcinoma and Its Association With the IL-6/TGF-ß1 Axis. J Surg Res 252:147-155 (2020). PubMed: 32278969
- Otsuru T et al. Epithelial-mesenchymal transition via transforming growth factor beta in pancreatic cancer is potentiated by the inflammatory glycoprotein leucine-rich alpha-2 glycoprotein. Cancer Sci 110:985-996 (2019). PubMed: 30575211
- Ban Z et al. LRG-1 enhances the migration of thyroid carcinoma cells through promotion of the epithelial-mesenchymal transition by activating MAPK/p38 signaling. Oncol Rep 41:3270-3280 (2019). PubMed: 31002347
- Li Z et al. Exosomal Leucine-Rich-Alpha2-Glycoprotein 1 Derived from Non-Small-Cell Lung Cancer Cells Promotes Angiogenesis via TGF-ß Signal Pathway. Mol Ther Oncolytics 14:313-322 (2019). PubMed: 31528707
- Gao Y et al. Mechanical strain promotes skin fibrosis through LRG-1 induction mediated by ELK1 and ERK signalling. Commun Biol 2:359 (2019). PubMed: 31602408
- Xiao S & Zhu H Leucine-Rich Alpha-2-Glycoprotein1 Gene Interferes with Regulation of Apoptosis in Leukemia KASUMI-1 Cells. Med Sci Monit 24:8348-8356 (2018). PubMed: 30455412
- Druhan LJ et al. Leucine Rich a-2 Glycoprotein: A Novel Neutrophil Granule Protein and Modulator of Myelopoiesis. PLoS One 12:e0170261 (2017). Functional Studies ; Human . PubMed: 28081565