Recombinant Anti-LRG1/LRG antibody [EPR12362] (ab178698)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-LRG1/LRG antibody [EPR12362]
See all LRG1/LRG primary antibodies -
Description
Rabbit monoclonal [EPR12362] to LRG1/LRG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
within Human LRG1/LRG aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P02750 -
Positive control
- Human plasma and Human serum lysates; Human lung tissue.
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General notes
This product was previously labelled as LRG1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12362 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab178698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Tissue specificity
Plasma. -
Sequence similarities
Contains 8 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 116844 Human
- Omim: 611289 Human
- SwissProt: P02750 Human
- Unigene: 655559 Human
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Alternative names
- 1300008B03Rik antibody
- 2310031E04Rik antibody
- A2GL antibody
see all
Images
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![Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)](https://www.abcam.com/ps/products/178/ab178698/Images/ab178698-206907-anti-lrg1-antibody-epr12362-western-blot.jpg)
Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698)All lanes : Anti-LRG1/LRG antibody [EPR12362] (ab178698) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human serum lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)](https://www.abcam.com/ps/products/178/ab178698/Images/ab178698-206908-anti-lrg1-antibody-epr12362-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (ab178698)Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LRG1/LRG with ab178698 at 1/100 dilution.
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![Immunoprecipitation - Anti-LRG1/LRG antibody [EPR12362] (ab178698)](https://www.abcam.com/ps/products/178/ab178698/Images/ab178698-206909-anti-lrg1-antibody-epr12362-immunoprecipitation.jpg)
Immunoprecipitation - Anti-LRG1/LRG antibody [EPR12362] (ab178698)Western blot analysis on immunoprecipitation pellet from (1) Human plasma lysate or (2) 1X PBS (negative control) immunoprecipitated using ab178698 at 1/10 dilution, with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Datasheets and documents
References
This product has been referenced in:
- Otsuru T et al. Epithelial-mesenchymal transition via transforming growth factor beta in pancreatic cancer is potentiated by the inflammatory glycoprotein leucine-rich alpha-2 glycoprotein. Cancer Sci 110:985-996 (2019). Read more (PubMed: 30575211) »
- Xiao S & Zhu H Leucine-Rich Alpha-2-Glycoprotein1 Gene Interferes with Regulation of Apoptosis in Leukemia KASUMI-1 Cells. Med Sci Monit 24:8348-8356 (2018). Read more (PubMed: 30455412) »
