Recombinant
RabMAb

Recombinant Anti-LRG1/LRG antibody [EPR12362] - BSA and Azide free (ab240209)

Overview

  • Product name

    Anti-LRG1/LRG antibody [EPR12362] - BSA and Azide free
    See all LRG1/LRG primary antibodies
  • Description

    Rabbit monoclonal [EPR12362] to LRG1/LRG - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human LRG1/LRG aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P02750

  • Positive control

    • WB: Human plasma and Human serum lysates;. IHC-P: human liver carcinoma and lung tissue.
  • General notes

    ab240209 is the carrier-free version of ab178698 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240209 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as LRG1

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Tissue specificity

      Plasma.
    • Sequence similarities

      Contains 8 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1300008B03Rik antibody
      • 2310031E04Rik antibody
      • A2GL antibody
      • A2GL_HUMAN antibody
      • HMFT1766 antibody
      • Leucine rich alpha 2 glycoprotein antibody
      • Leucine-rich alpha-2-glycoprotein 1 antibody
      • Leucine-rich alpha-2-glycoprotein antibody
      • Leucine-rich alpha-2-glycoprotein precursor antibody
      • LRG antibody
      • LRG1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling LRG1/LRG with purified ab178698 at 1/200 dilution (0.57 µg/ml). Heat mediated antigen retrieval using Bond� Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178698)
    • Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LRG1/LRG with ab178698 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178698).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab240209 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240209.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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