Key features and details
- Mouse monoclonal [LRG3-3] to LRIG3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-LRIG3 antibody [LRG3-3]
See all LRIG3 primary antibodies
DescriptionMouse monoclonal [LRG3-3] to LRIG3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Monkey
- U87 cells; H1395 cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.098% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab213960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 123 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionMay play a role in craniofacial and inner ear morphogenesis during embryonic development. May act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1.
Tissue specificityWidely expressed.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 15 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Cellular localizationCell membrane. Cytoplasmic vesicle membrane. Detected in cytoplasmic vesicles when coexpressed with ERBB4.
- Information by UniProt
- Leucine-rich repeats and immunoglobulin-like domains protein 3 antibody
- Leucine-rich repeats- and immunoglobulin-like domains- containing protein 3 antibody
- LIG-3 antibody
Immunofluorescent analysis of U87 cells (4% paraformaldehyde fixed, 0.5% Triton X-100 permealized) labeling LRIG3 with ab213960 at 5 μg/ml dilution, followed by Goat Anti-Mouse IgG, Cy3 conjugate secondary detection (red). Cells were counterstained with DAPI (blue) to stain nuclei.
All lanes : Anti-LRIG3 antibody [LRG3-3] (ab213960)
Lane 1 : H1395 cell extract
Lane 2 : H1395 cell extract with LRIG3 immunizing peptide (Human, 65-82)
Lysates/proteins at 2 µg/ml per lane.
All lanes : Goat Anti-Mouse IgG-Peroxidase
Developed using the ECL technique.
Predicted band size: 123 kDa
ab213960 has been referenced in 1 publication.
- Paran N et al. Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication. Cell Host Microbe 6:563-9 (2009). PubMed: 20006844